Inhalation vs. Aspiration of Single-Walled Carbon Nanotubes in C57BL/6 Mice: Inflammation, Fibrosis, Oxidative Stress, and Mutagenesis

Details

• Personal Author:
  Arepalli S ; Baron, Paul A. ; Castranova, Vincent ; Chen BT ; Deye G ; Gorelik O ; Hubbs, Ann F. ; Jin J ; Johnson VJ ; Kagan VE ; Keohavong P ; Kisin E ; Maynard A ; Mercer RR ; Murray AR ; Shvedova AA ; Stone S ; Sussman N ; Yin J
• Description:
  Nanomaterials are frontier technological products used in different manufactured goods. Because of their unique physicochemical, electrical, mechanical, and thermal properties, single-walled carbon nanotubes (SWCNT) are finding numerous applications in electronics, aerospace devices, computers, and chemical, polymer, and pharmaceutical industries. SWCNT are relatively recently discovered members of the carbon allotropes that are similar in structure to fullerenes and graphite. Previously, we have reported that pharyngeal aspiration of purified SWCNT by C57BL/6 mice caused dose-dependent granulomatous pneumonia, oxidative stress, acute inflammatory/cytokine responses, fibrosis, and decrease in pulmonary function. To avoid potential artifactual effects due to instillation/agglomeration associated with SWCNT, we conducted inhalation exposures using stable and uniform SWCNT dispersions obtained by a newly developed aerosolization technique (2). The inhalation of nonpurified SWCNT (iron content of 17.7% by weight) at 5 mg/m3, 5 h/day for 4 days was compared with pharyngeal aspiration of varying doses (5-20 µg per mouse) of the same SWCNT. The chain of pathological events in both exposure routes was realized through synergized interactions of early inflammatory response and oxidative stress culminating in the development of multifocal granulomatous pneumonia and interstitial fibrosis. SWCNT inhalation was more effective than aspiration in causing inflammatory response, oxidative stress, collagen deposition, and fibrosis as well as mutations of K-ras gene locus in the lung of C57BL/6 mice. [Description provided by NIOSH]
• Subjects:
  Animals Fibrosis Laboratories Occupational Health Respiratory Tract Diseases Safety
• Keywords:
  Animal-studies In-vitro-studies Laboratory-animals Nanotechnology Pulmonary-system-disorders Respiratory-system-disorders
• ISSN:
  1040-0605
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  District of Columbia ; Ohio ; OSHA Region 3 ; OSHA Region 5 ; OSHA Region 6 ; Pennsylvania ; Texas ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  295
• Issue:
  4
• NIOSHTIC Number:
  nn:20034576
• Citation:
  Am J Physiol Lung Cell Mol Physiol 2008 Oct; 295(4):L552-L565
• Contact Point Address:
  A. A. Shvedova, Health Effects Laboratory Division, NIOSH, Morgantown, WV 26505
• Email:
  AShvedova@cdc.gov
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  American Journal of Physiology: Lung Cellular and Molecular Physiology
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:17d828bca63743ba8402451b7cbb9dd926f9a3bea12a3f30ebd84acb24e4a464b696910fa4e5cda767428101f08e252ec13bdad8ebdbf17cf13e0968447be9a2
• Download URL:
  https://stacks.cdc.gov/view/cdc/186735/cdc_186735_DS1.pdf
• File Type:
  [PDF - 1.16 MB ]