Lifetime Persistence and Clonality of Chromosome Aberrations in the Peripheral Blood of Mice Acutely Exposed to Ionizing Radiation

Details

• Personal Author:
  Nath J ; Nelson DO ; Ramsey MJ ; Spruill MD ; Tucker JD
• Description:
  As the measurement of chromosomal translocations increases in popularity for quantifying prior radiation exposure, information on the possible decline of these "stable" aberrations over time is urgently needed. We report here information about the persistence of radiation-induced chromosome aberrations in vivo over the life span of a rodent. Female C57BL/6 mice were given a single whole-body acute exposure of 0, 1, 2, 3 or 4 Gy Cs-137 gamma rays at 8 weeks of age. Chromosome aberrations were analyzed from peripheral blood samples at various intervals between 1 day and 21 months after exposure. Aberrations were detected by painting chromosomes 2 and 8, Translocations decreased dramatically during the first 3 months after irradiation, beyond which time the frequencies remained relatively constant out to 1 year, when the effects of aging and clonal expansion became significant. Both reciprocal and nonreciprocal translocations increased with age in the unexposed control animals and were involved in clones. As expected of unstable aberrations, dicentrics decreased rapidly after exposure and reached baseline levels within 3 months. These results indicate that the persistence of translocations induced by ionizing radiation is complicated by aging and clonal expansion and that these factors must be considered when quantifying translocations at long times after exposure. These results have implications for biological dosimetry in human populations. [Description provided by NIOSH]
• Subjects:
  Age Factors Biological Factors Blood Chromosomes Genetics Hematologic Tests Occupational Health Radiation Radiation Dosimeters Safety Sex Characteristics

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• Keywords:
  Age-factors Biological-effects Biological-factors Biological-transport Blood-analysis Blood-samples Blood-tests Chromosome-disorders Dosimetry Genotoxic-effects Radiation-effects Radiation-exposure Radiation-measurement Rodents Sex-factors

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• ISSN:
  0033-7587
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  110-121
• Volume:
  153
• Issue:
  1
• NIOSHTIC Number:
  nn:20032856
• Citation:
  Radiat Res 2000 Jan; 153(1):110-121
• Contact Point Address:
  James D. Tucker, Biology and Biotechnology Research Program, L-452, P.O. Box 808, Lawrence Livermore National Laboratory, Livermore, California 94551
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  True
• Source Full Name:
  Radiation Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4eb4fd9d206b38897b8276f997d0ea15530eccdb094161648fbd23276f79d4b4e161fc8ce785c30b155378705e9c848785e3946ed2d1198c38fbd4c62d4debb6
• Download URL:
  https://stacks.cdc.gov/view/cdc/186669/cdc_186669_DS1.pdf
• File Type:
  [PDF - 289.25 KB ]