TLR4 and Inflammatory Response to Neuronal Degeneration

Details

• Personal Author:
  Konat GW ; Kraszpulski M ; O'Callaghan JP ; Sriram K
• Description:
  Toll-like receptor 4 (TLR4) has recently emerged as an important mediator of inflammatory responses elicited by molecules released from injured tissues. The present study was undertaken to determine the role of TLR4 in such responses following neuronal damage. Striatal dopaminergic neurodegeneration was induced in TLR4-mutant and control mice by a single subcutaneous injection of 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP). In both groups, the level of tyrosine hydroxylase (TH) in the striatum decreased within 72 h by B50% as compared with untreated animals indicating extensive neuronal damage. The loss of TH was associated with glial activation as seen from the up-regulation of GFAP, which was similar in both groups. The phosphorylation of STAT3, a key mediator of astrogliosis, was also increased. Moreover, the expression of glia-derived cytokines, i.e., TNFa, MCP-1 and LIF, was profoundly up-regulated in both mutant and control striata. Interestingly, basal expression of MCP-1 and LIF in TLR4-mutants was approximately five fold higher than in controls. These results indicate that TLR4 does not mediate inflammatory responses associated with dopaminergic neurodegeneration. However, the receptor seems to be involved in controlling basal expression of certain inflammatory genes. [Description provided by NIOSH]
• Subjects:
  Animals Genetics Laboratories Nervous System Nervous System Diseases Occupational Health Safety
• Keywords:
  Animal-studies Gene-mutation Genes Genotoxic-effects Genotoxicity Laboratory-animals Neuromotor-disorders Neuromuscular-system-disorders Tissue-disorders
• ISSN:
  0022-3042
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  137-138
• Volume:
  102
• NIOSHTIC Number:
  nn:20032695
• Citation:
  J Neurochem 2007 Aug; 102(Suppl 1):137-138
• Contact Point Address:
  NIOSH, CDCP, 1095 Willowdale Rd, MS 2800, Morgantown, WV 26505
• Federal Fiscal Year:
  2007
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Neurochemistry
• Supplement:
  1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:42589f71f858ed1ecf45b315976752b001d23423fe61adf93ac4f68fa21b1816e386a0ccbd5ae4818c466a3c077d1b9e65e9001385b074ee42733a63529bc27d
• Download URL:
  https://stacks.cdc.gov/view/cdc/186577/cdc_186577_DS1.pdf
• File Type:
  [PDF - 86.16 KB ]