A Novel Nonphagocytic Mechanism of Erythrocyte Destruction Involving Direct Cell-Mediated Cytotoxicity

Details

• Personal Author:
  Chandrasekhar B ; Saxena RK
• Description:
  The number of erythrocytes fell when co-cultured with cell preparations derived from mouse spleen, thymus, bone marrow, or peritoneal exudate (PE) cells. Erythrocyte-depletion activities (EDA) of different leukocyte preparations were in the order PE > spleen > thymus > bone marrow. Adherent, nonadherent, T-depleted, and T-enriched cell subpopulations had comparable EDA. Spleen cells from athymic nude mice, however, lacked significant EDA. In addition, EDA was boosted by Concanavalin A (Con A) but not by lipopolysaccharide, indicating that T cells may play a crucial role in inducing EDA in spleen cells. Paraformaldehyde-fixed spleen or PE cells, as well as membrane preparations isolated from spleen cells, efficiently lysed erythrocytes. Erythrocyte ghost membranes inhibited erythrocyte lysis by control or paraformaldehyde-fixed spleen cells. Treatment with hamster anti-mouse Fas or anti-mouse tumor necrosis factor receptor (TNFR) antibody could opsonize erythrocytes for faster depletion by spleen cells, suggesting an expression of Fas and TNFR on erythrocytes. TNF alpha could lyse erythrocytes in a dose-dependent fashion. Additionally, enhanced spleen cell EDA induced in response to succenyl Con-A could be blocked by anti-TNF alpha antibodies. Our results provide evidence for a direct cell-mediated cytotoxicity (CMC) of erythrocytes by leukocytes. A role of molecules of Fas and the TNF family in CMC of erythrocytes has also been suggested. Further work is needed to understand if, and to what extent, CMC of erythrocytes contributes to erythrocyte destruction in vivo and to determine its patho-physiological significance. [Description provided by NIOSH]
• Subjects:
  Animals Occupational Health Safety
• Keywords:
  Analytical-processes Animal-studies Cell-alteration Cell-biology Cell-damage Cell-function Cell-metabolism Cell-transformation Cellular-reactions
• ISSN:
  0925-5710
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  227-237
• Volume:
  71
• Issue:
  3
• NIOSHTIC Number:
  nn:20032674
• Citation:
  Int J Hematol 2000 Apr; 71(3):227-237
• Contact Point Address:
  RK Saxena, NIOSH, ASB, 1095 Willowdale Rd, Morgantown, WV 26505
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  True
• Source Full Name:
  International Journal of Hematology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:64d2be8ffc172c2db8ad5b6113cc860872ed4c74810c9ba654b3126dcb5f89a5fd7228473fba9ddef9414485ade102bbdfa5765a09b687b27a170d370ade1fb4
• Download URL:
  https://stacks.cdc.gov/view/cdc/186563/cdc_186563_DS1.pdf
• File Type:
  [PDF - 1.21 MB ]