The Release of Reactive Oxidant Species by FMLP-Stimulated Leukocytes Increases Microvessel Permeability

Details

• Personal Author:
  Castranovo V ; He P ; Zhu L
• Description:
  Our previous study demonstrated that systemic application of TNF-alpha induced a significant leukocyte adhesion without increasing microvessel permeability. Our present study is to investigate the effect of fMLP-stimulated leukocytes on microvessel permeability. The ROS production upon neutrophil activation was quantified by measuring the changes in chemiluminescence (CL) using a luminometer. The peak CL signal increased 3.1 fold after exposure of isolated rat neutrophils (2 x 10(6)/ml) to fMLP (10 µM). Measurements of hydraulic conductivity (Lp) in single perfused rat mesenteric venular microvessels showed that perfusing the vessel with a fMLP-stimulated neutrophil suspension (2 x 10(7)/ml) increased Lp to 3.7 +/- 0.6 times the control (n=4). After adding fMLP (10 µM) to the perfusate (n=4) or superfusate (n=8) and perfusing the vessel under balance pressure for 5 min in the presence of TNF-alpha-induced adherent leukocytes (14 +/- 1/100 µm), the mean peak Lp increased to 5.2 +/- 0.9 and 4.6 +/- 0.5 times control, respectively. The application of DFO, an iron chelator, attenuated the Lp increase to 1.5 +/- 0.2 times control (n=4). In contrast, neither TNF-alpha-induced leukocyte adhesion nor perfusing fMLP alone changed basal Lp. These results suggest that the fMLP-stimulated leukocytes increase microvessel permeability by increased ROS production, which is independent of leukocyte adhesion and migration processes. [Description provided by NIOSH]
• Subjects:
  Chelating Agents Genetics Microbiology Occupational Health Safety
• Keywords:
  Cell-wall-permeability Chelating-agents Leukocytes Neutrophils Oxidative-processes
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  17
• Issue:
  4
• NIOSHTIC Number:
  nn:20032638
• Citation:
  FASEB J 2003 Mar; 17(4)(Pt 1)(Suppl S):A137
• Contact Point Address:
  West Virginia University, Health Science Center, Morgantown, WV 26506
• Federal Fiscal Year:
  2003
• Peer Reviewed:
  False
• Part Number:
  1
• Source Full Name:
  The FASEB Journal
• Supplement:
  S
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1ce4251c711f98db098ae129357befe44ad40f6a22824b24b0ffaca2ae554e80f1d40c1847ab49e2a67e5c8491ce0d58675bd9f076fb4a7bc62f803d025d1c12
• Download URL:
  https://stacks.cdc.gov/view/cdc/186537/cdc_186537_DS1.pdf
• File Type:
  [PDF - 223.81 KB ]