Lab-on-a-Chip Methods for Point-of-Care Measurements of Salivary Biomarkers of Periodontitis

Details

• Personal Author:
  Ballard KLM ; Christodoulides N ; Dharshan P ; Ebersole JL ; Floriano PN ; Griffin M ; King CP Jr. ; Kryscio RJ ; Langub MC ; Lennart A ; McDevitt JT ; Miller CS ; Mohanty S ; Thomas MV ; Ballard KLM ; Christodoulides N ; Dharshan P ; Ebersole JL ; Floriano PN ; Griffin M ; King CP Jr. ; Kryscio RJ ; Langub MC ; Lennart A ; McDevitt JT ; Miller CS ; Mohanty S ; Thomas MV
• Description:
  Salivary secretions contain a variety of molecules that reflect important pathophysiological activities. Quantitative changes of specific salivary biomarkers could have significance in the diagnosis and management of both oral and systemic diseases. Modern point-of-care technologies with enhanced detection capabilities are needed to implement a significant advancement in salivary diagnostics. One such promising technology is the recently described lab-on-a-chip (LOC) assay system, in which assays are performed on chemically sensitized beads populated into etched silicon wafers with embedded fluid handling and optical detection capabilities. Using this LOC system, complex assays can be performed with small sample volumes, short analysis times, and markedly reduced reagent costs. This report describes the use of LOC methodologies to assess the levels of interleukin-1 beta (IL-1 beta), C-reactive protein (CRP), and matrix metalloproteinase-8 (MMP-8) in whole saliva, and the potential use of these biomarkers for diagnosing and categorizing the severity and extent of periodontitis. This study demonstrates that the results achieved by the LOC approach are in agreement with those acquired with standard enzyme-linked immunosorbent assay (ELISA), with significant IL-1 beta and MMP-8 elevations in whole saliva of periodontitis patients. Furthermore, because of the superior detection capacities associated with the LOC approach, unlike those with ELISA, significant differences in CRP levels between periodontitis patients and normal subjects are observed. Finally, principal component analysis (PCA) is performed to yield an efficient method to discriminate between periodontally healthy and unhealthy patients, thus increasing the diagnostic value of these biomarkers for periodontitis when examined with the integrated LOC sensor system. [Description provided by NIOSH]
• Subjects:
  Anatomy Occupational Health Safety
• Keywords:
  Analytical-Method Analytical-processes Dental-health Enzyme-complexes Periodontal-disorders Salivary-gland-disorders Salivary-glands
• ISSN:
  0077-8923
• Document Type:
  Text
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  Georgia ; Kentucky ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 6 ; Texas ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  OD - Office of the Director ; OD - Office of the Director
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Pages in Document:
  411-428
• Volume:
  1098
• NIOSHTIC Number:
  nn:20032415
• Citation:
  Ann NY Acad Sci 2007 Mar; 1098:411-428
• Contact Point Address:
  Dr. John T. McDevitt, University of Texas at Austin, Department of Chemistry and Biochemistry, 1 University Station - A5300, Austin, TX 78712
• Email:
  mcdevitt@mail.utexas.edu
• Federal Fiscal Year:
  2007
• Peer Reviewed:
  True
• Source Full Name:
  Annals of the New York Academy of Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e97d540009d586669f98544f1389f357dd9e059ab776230815abfa417b3f8d7a5e695f6e029c1d563c5a1126b3a1a8f7373848d48df636a0ea6249abce7087df
• Download URL:
  https://stacks.cdc.gov/view/cdc/186399/cdc_186399_DS1.pdf
• File Type:
  [PDF - 259.17 KB ]