Effect of Antioxidant Protection by P-Coumaric Acid on Low-Density Lipoprotein Cholesterol Oxidation

Details

• Personal Author:
  Castranova, Vincent ; Cosma G ; Gardner H ; Shi XL ; Vallyathan V ; Zang LY
• Description:
  Mechanisms in which p-coumaric acid (CA) acts as an antioxidant are not well understood. This study investigated whether CA can act as a direct scavenger of reactive oxygen species (ROS) and whether it minimizes the oxidation of low-density lipoprotein (LDL). Rats were administered CA in drinking water at low or high doses for 10, 21, and 30 days (uptakes were 29 and 317 mg/day, respectively). Blood levels of 8-epiprostaglandin F-2 alpha were monitored as a marker of LDL oxidation. Oral administration of CA (317 mg/day) for 30 days significantly inhibited LDL oxidation. CA also reduced LDL cholesterol levels in serum but had no effect on levels of high-density lipoprotein cholesterol. In vitro studies that used electron spin resonance in combination with spin trapping techniques were used to determine the ability of CA to scavenge ROS and alter LDL oxidation. CA effectively scavenged . OH in a dose-dependent manner. IC50 and maximum velocity for CA scavenging of . OH were 4.72 mu M and 1.2 mu M/s, respectively, with a rate constant of 1.8 X 10(11) M-1.s(-1). Our studies suggest that the antioxidant properties of CA may involve the direct scavenging of ROS such as OH. [Description provided by NIOSH]
• Subjects:
  Animals Lipids Occupational Health Safety
• Keywords:
  Analytical-Method Analytical-processes Animal-studies Author Keywords: Hydroxyl Radical Lipid-peroxidation Lipid Peroxidation Mathematical-models Oxidation Oxidative-processes Reactive Oxygen Species Standards Statistical-analysis

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• ISSN:
  0363-6143
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; Maryland ; OSHA Region 3 ; OSHA Region 8 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  279
• Issue:
  4
• NIOSHTIC Number:
  nn:20032347
• Citation:
  Am J Physiol Cell Physiol 2000 Oct; 279(4):C954-C960
• Contact Point Address:
  V Vallyathan, Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Rd., Morgantown, WV 26505-2888
• Email:
  vav1@cdc.gov
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  American Journal of Physiology: Cell Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1fcafab26d09e2a0e74c0fb190159a67e1955b6331b9856315367dc0f778162f85b3bd57970150145943a8b45db7af3550b1544536f13e0557500736af28d992
• Download URL:
  https://stacks.cdc.gov/view/cdc/186363/cdc_186363_DS1.pdf
• File Type:
  [PDF - 212.32 KB ]