Free Radical-Mediated Transgene Inactivation of Macrophages by Endotoxin

Details

• Personal Author:
  Dokka S ; Huang CS ; Leonard, Stephanie ; Rojanasakul Y ; Shi XL ; Toledo D ; Wang LY
• Description:
  Endotoxin, the lipopolysaccharide component of gram-negative bacteria, is a common contaminant of plasmid DNA preparations. The present study investigated the effect of endotoxin on gene transfection efficiency and the role of reactive oxygen species (ROS) in this process. Gene transfection studies were performed in various cell types with cytomegalovirus-luciferase as a reporter plasmid and cationic liposome as a transfecting agent. The presence of endotoxin in plasmid DNA preparations severely limited transgene expression in macrophages but had little or no effect in other cell types tested. This decreased transfection was dependent on ROS-mediated cellular toxicity induced by endotoxin. Neutralizing the endotoxin by the addition of polymyxin B effectively increased transfection efficiency and reduced toxicity. Electron spin resonance studies confirmed the formation of ROS in endotoxin-treated cells and their inhibition by free radical scavengers. The ROS scavenger N-t-butyl-alpha -phenylnitrone, the H2O2 scavenger catalase, and the OH scavenger sodium formate effectively inhibited endotoxin-induced effects, whereas the O-2(-) scavenger superoxide dismutase had lesser effects. These results indicate that multiple oxidative species are involved in the transfection inactivation process and that OH formed by H2O2-dependent, metal-catalyzed Fenton reaction play a major role in this process. [Description provided by NIOSH]
• Subjects:
  Endotoxins Genetics Occupational Health Safety
• Keywords:
  Analytical-methods Analytical-processes Author Keywords: Gene Transfection Cell-biology Cell-metabolism Cell-transformation Free Radicals Genes Genetic-factors Genotoxic-effects Macrophages Oxidative-processes

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• ISSN:
  1040-0605
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  279
• Issue:
  5
• NIOSHTIC Number:
  nn:20032337
• Citation:
  Am J Physiol Lung Cell Mol Physiol 2000 Nov; 279(5):L878-L883
• Contact Point Address:
  Y Rojanasakul, West Virginia Univ. School of Pharmacy, Dept. of Basic Pharmaceutical Sciences, PO Box 9530, Morgantown, WV 26506
• Email:
  yrojanasakul@hsc.wvu.edu
• CAS Registry Number:
  Sodium formate (CAS RN 141-53-7)
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  American Journal of Physiology: Lung Cellular and Molecular Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3815afdd87a3b7d8ed073c413a068cb6fcb3ad839a8d7f9b48468f343ddad356fe43745225532244772c2bd31722e8940926eca1086a4301811f58fce6dcc4a7
• Download URL:
  https://stacks.cdc.gov/view/cdc/186355/cdc_186355_DS1.pdf
• File Type:
  [PDF - 172.27 KB ]