Relationship Between Formation of Carcinogen-DNA Adducts [CA] and Micronuclei [MN] in Mouse Keratinocytes Exposed to Carcinoges

Details

• Personal Author:
  Krishnan SP ; Warshawsky D
• Description:
  This project is concerned with determining the relationship between two biological markers of exposure [CA and MN] in viable keratinocytes following exposure to an environmental and occupational carcinogen; 7H-dibenzo[c,g]carbazole [DBC], in comparison to the carcinogen-7, 12­ dimethylbenz[a]anthracene [DMBA]. Mouse keratinocytes were grown on collagen coated dishes [CA analysis] and slides [MN analysis] and dosed with the carcinogen. The dishes were trypsinized and the cells kept frozen at -80 degrees C for subsequent DNA isolation and CA analysis [32 P-postlabelling]. The medium for the cells grown on slides was replaced with medium contain­ing cytochalasin-B (drug which blocks cytokinesis 3 j,tglml) and the cells were cultured for 72 hours, then fixed in 100% methanol and stained with acridine orange. DBC induced a dose-dependent [10-1000 ngtml] increase in MN [36.2-105.8 MN/1000 binucleated [BN] cells] over solvent controls [32 +/- 3.7; N = 3] and in CA [10.9-37 (Relative Adduct Labelling [RAL] x 107 nucleotides) over solvent controls [5.7 +/- 2.9 (RAL x 107 nucleotides); N = 4]. DMBA induced a dose-dependent [32-256 ng/ml] increase in MN [46.6­110.1 MN/l000 BN cells] over solvent controls [21.3 +/- lQ.4; N = 3]. DMBA­induced CA [3.5-10.8 (RAL x 107 nucleotides)] were very low when compared to solvent controls [2.4 +/- 0.7; N = 3]. The results indicate that the relation­ship between MN frequency and CA formation is unique for each compound tested. [Description provided by NIOSH]
• Subjects:
  Animals Biomarkers Carcinogens Environmental Monitoring Laboratories Neoplasms Occupational Health Safety
• Keywords:
  Animal-studies Cancer Cell-morphology Cell-transformation DNA-adducts Exposure-assessment Laboratory-animals
• ISSN:
  0731-9193
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  81
• Volume:
  15
• Issue:
  1
• NIOSHTIC Number:
  nn:20032266
• Citation:
  Toxicologist 1995 Mar; 15(1):81
• Federal Fiscal Year:
  1995
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  False
• Start Date:
  19910930
• Source Full Name:
  The Toxicologist. Society of Toxicology 34th Annual Meeting, March 5-9,1995, Baltimore, Maryland
• End Date:
  19940929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c597b477304997051a9fa865a62ecd9e265e778491e97b39b152dbb5b63b258afdc4563c3e6070f2533cb8853fbf190449c8da3a8903b8b44f06ca08cab79282
• Download URL:
  https://stacks.cdc.gov/view/cdc/186297/cdc_186297_DS1.pdf
• File Type:
  [PDF - 894.52 KB ]