Systemic Changes in Arginase and Arginine Metabolism in a Model of Atherosclerosis: A Comparison of apoE-/- and C57 Mice

Details

• Personal Author:
  Chapman R ; Erdely A ; Hulderman T ; Kepka-Lenhart D ; Morris SM ; Salmen R ; Simeonova P
• Description:
  Increased arginase activity is implicated in cardiovascular pathogenesis. Therefore we studied systemic changes in arginase and arginine metabolism in a model of atherosclerosis. ApoE-/- and C57 mice were placed on 45 or 60% fat diets for 2 months. Results showed increased circulating arginase activity with no difference between 45% (14-fold) and 60% (16-fold) fat diet in the apoE-/-. There was a mild increase in the C57 with 45% fat (2.4-fold) and a large increase due to 60% (19-fold). In the plasma, circulating arginine was reduced in the apoE-/- and showed a decreasing trend in the C57. The plasma arginine/ornithine ratio was significantly decreased in all groups that showed profound increased plasma arginase activity. Markers of tissue (LDH) and liver (ALT) injury were markedly elevated in mice with increased arginase activity. Interestingly, blood cell gene expression in the apoE-/- fed 60% fat showed an 11-fold increase in arginase II (AII) and no increase in arginase I (AI). Tissue gene expression showed increased AI (20- 5.5-fold) and AII (14- 19-fold) in aorta and heart respectively in 60% fat apoE-/- (45% to be completed) with no increase in C57. In conclusion, increased circulating arginase activity and reduced arginine are likely the result of liver and/or tissue injury. Increased arginase gene expression in the blood and tissue (apoE-/- only) are likely associated with the development of atherosclerosis. [Description provided by NIOSH]
• Subjects:
  Animals Blood Blood-Borne Pathogens Cardiovascular Diseases Cardiovascular System Genetics Hematologic Diseases Liver Occupational Health Safety
• Keywords:
  Animal-studies Blood-cells Blood-plasma Blood-vessels Bloodborne-pathogens Cardiac-function Cardiovascular-function Cardiovascular-system-disorders Cell-biology Cell-differentiation Cell-function Cellular-function Cellular-structures Genes Liver-cells Liver-disorders Liver-tissue Tissue-disorders

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• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  21
• Issue:
  6
• NIOSHTIC Number:
  nn:20031958
• Citation:
  FASEB J 2007 Apr; 21(6)(Suppl):A1404
• Contact Point Address:
  Health Effects Laboratory Division, CDC National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV, 26505
• Federal Fiscal Year:
  2007
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal
• Supplement:
  Suppl
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:42ebdf635f6284a2de756eda72ac59fa6f622c61bc62af8f104765f040e2e6850ba7877a8f63c2d08938d1ce2952c63b47c3d90d57ecee21ba94ce703cdf903f
• Download URL:
  https://stacks.cdc.gov/view/cdc/186065/cdc_186065_DS1.pdf
• File Type:
  [PDF - 404.88 KB ]