Phosphatidylserine Enhances Recognition and Uptake of Single-Walled Carbon Nanotubes by Rat Brain Microglial Cells

Details

• Personal Author:
  Bayir H ; Feng W ; Kagan VE ; Shvedova AA ; Tyurina YY
• Description:
  Single-walled carbon nanotubes (SWCNT) are a class of nanosized materials with wide applications in industry and medicine. Inhaled SWCNT have been shown to cause a robust inflammatory and oxidative stress response in the lung and cardiovascular system. Interactions with phagocytozing cells determine SWCNT elimination or spreading into different tissues. The challenge is how to make nanotubes delivered specifically to the target site. We established that macrophages do not effectively recognize non-functionalized SWCNT but readily engulf SWCNT coated with phosphatidylserine (PS), an "eat-me" signal for phagocytes. In the brain, important immunological functions are associated with the ability of microglial cells to exert phagocytic activity. As inhaled nanoparticles have been detected in the brain we were interested to study interactions of SWCNT with microglial cells. Microglial cells were isolated from postnatal rat brains and identified with CD11 antibody. Primary cultures were obtained with 70% microglial cell population which could be activated by lipopolysacharide (LPS) to produce NO revealed by labeling of cells with a specific fluorogenic reagent, DAF-2DA. Further we studied phagocytosis of SWCNT by microglial cells. Cells were incubated with SWCNT coated with either fluorescently labeled nitrobenzoxadiazole (NBD)-PS or NBD-phosphatidylcholine (PC) for 2h. Fluorescent microscopy was applied and revealed that NBD-PS-SWCNT were a more preferred target of recognition and uptake by microlgial cells than NBD-PC-SWCNT. Control experiments were performed to confirm that NBD-phospholipids remained bound to SWCNT. Thus, similar to macrophages, microglial cells utilize PS as a signal for effective recognition and engulfment of SWCNT. [Description provided by NIOSH]
• Subjects:
  Animals Microbiology Occupational Health Oxidants Respiratory System Safety
• Keywords:
  Animal-studies Cell-alteration Cell-biology Cell-damage Cell-function Cell-metabolism Cellular-reactions Inhalation-studies Microchemistry Microscopic-analysis Nanotechnology Oxidation Oxidative-metabolism Oxidative-processes

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• ISSN:
  1096-6080
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  96
• Issue:
  1
• NIOSHTIC Number:
  nn:20031929
• Citation:
  Toxicologist 2007 Mar; 96(1):289
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2007
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  False
• Start Date:
  20050701
• Source Full Name:
  The Toxicologist. Society of Toxicology 46th Annual Meeting and ToxExpo, March 25-29, 2007, Charlotte, North Carolina
• End Date:
  20150630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5e691ed0aedb628e1660539ba8292c989d954c2b2b3e0288e5cf217c7f7474f46a62c71a160d517f2ed8a904e0eb448b5e37358fcedd30b07afe2937d696c7cc
• Download URL:
  https://stacks.cdc.gov/view/cdc/186039/cdc_186039_DS1.pdf
• File Type:
  [PDF - 171.37 KB ]