Cardiovascular Effects of Pulmonary Exposure to Single-Wall Carbon Nanotubes

Details

• Personal Author:
  Chapman R ; Hulderman T ; Leonard, Stephen S. ; Li Z ; Luster MI ; Salmen R ; Shvedova A ; Simeonova PP ; Young SH ; Chapman R ; Hulderman T ; Leonard, Stephen S. ; Li Z ; Luster MI ; Salmen R ; Shvedova A ; Simeonova PP ; Young SH
• Description:
  Background: Engineered nanosized materials, such as single-wall carbon nanotubes (SWCNT), are emerging as technologically important in different industries. Objective: The unique physical characteristics and the pulmonary toxicity of SWCNTs raised concerns that respiratory exposure to these materials may be associated with cardiovascular adverse effects. Methods: In these studies we evaluated aortic mitochondrial alterations by oxidative stress assays, including quantitative polymerase chain reaction of mitochondrial (mt) DNA and plaque formation by morphometric analysis in mice exposed to SWCNTs. Results: A single intrapharyngeal instillation of SWCNTs induced activation of heme oxygenase-1 (HO-1), a marker of oxidative insults, in lung, aorta, and heart tissue in HO-1 reporter transgenic mice. Furthermore, we found that C57BL/6 mice, exposed to SWCNT (10 and 40 µg/mouse) , developed aortic mtDNA damage at 7, 28, and 60 days after exposure. mtDNA damage was accompanied by changes in aortic mitochondrial glutathione and protein carbonyl levels. Because these modifications have been related to cardiovascular diseases, we evaluated whether repeated exposure to SWCNTs (20 µg/mouse once every other week for 8 weeks) stimulates the progression of atherosclerosis in ApoE-/- transgenic mice. Although SWCNT exposure did not modify the lipid profiles of these mice, it resulted in accelerated plaque formation in ApoE-/- mice fed an atherogenic diet. Plaque areas in the aortas, measured by the en face method, and in the brachiocephalic arteries, measured histopathologically, were significantly increased in the SWCNT-treated mice. This response was accompanied by increased mtDNA damage but not inflammation. Conclusions: Taken together, the findings are of sufficient significance to warrant further studies to evaluate the systemic effects of SWCNT under workplace or environmental exposure paradigms. [Description provided by NIOSH]
• Subjects:
  Aerosols Animals Cardiovascular Diseases Cardiovascular System Dust Laboratories Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Aerosol-particles Animal-studies Cardiopulmonary-system-disorders Cardiovascular-disease Cardiovascular-system Cardiovascular-system-disease Cardiovascular-system-disorders Laboratory-animals Nanotechnology Respirable-dust
• ISSN:
  0091-6765
• Document Type:
  Text
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Pages in Document:
  377-382
• Volume:
  115
• Issue:
  3
• NIOSHTIC Number:
  nn:20031709
• Citation:
  Environ Health Perspect 2007 Mar; 115(3):377-382
• Contact Point Address:
  P. Simeonova, Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 USA
• Email:
  PSimeonova@cdc.gov
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0) ; Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2007
• Peer Reviewed:
  True
• Source Full Name:
  Environmental Health Perspectives
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1d43cba2f3c3cdb6800bf0946fe9edb0e3aaf546968765cf764b083fbb4f2a755e8dec9248bba2765b632ed990c936b3a48ad4db56e1a2962417ada2d6d1a1fb
• Download URL:
  https://stacks.cdc.gov/view/cdc/185907/cdc_185907_DS1.pdf
• File Type:
  [PDF - 656.87 KB ]