Cell- and Isoform-Specific Increases in Arginase Expression in Acute Silica-Induced Pulmonary Inflammation

Details

• Personal Author:
  Beighley C ; Castranova, Vincent ; Kepka-Lenhart D ; Millecchia L ; Morris SM Jr. ; Poljakovic M ; Porter DW ; Wolfarth MG
• Description:
  Arginase induction was reported in several inflammatory lung diseases, suggesting that this may be a common feature underlying the pathophysiology of such diseases. As little is known regarding arginase expression in silicosis, the induction and cellular localization of arginase were elucidated in lungs of Sprague-Dawley rats 24 h following exposure to varying doses of silica by intratracheal instillation. Arginase expression was evaluated by activity assay, quantification of arginase I and arginase II mRNA levels using real-time polymerase chain reaction (PCR), and immunohistochemistry. Analyses of cells and fluid obtained by bronchoalveolar lavage (BAL) showed that markers of pulmonary inflammation, tissue damage, activation of alveolar macrophages (AM) and NO production were significantly increased by all silica doses. Arginase activity was increased also in AMs isolated from BAL fluid of silica-treated rats. Silica produced two- and three-fold increases in arginase activity of whole lung at doses of 1 and 5 mg/100 g body weight, respectively. Levels of arginase I mRNA, but not of arginase II mRNA, were similarly elevated. In control lungs, arginase I immunoreactivity was observed only in AMs sparsely dispersed throughout the lung; no inducible nitric oxide synthase (iNOS) immunoreactivity was detected. In silica-treated lungs, arginase I and iNOS were co-expressed in most AMs that were abundantly clustered at inflammatory foci. The rapid induction of arginase I expression in inflammatory lung cells, similar to induction of arginase in other inflammatory lung diseases, implicates elevated arginase activity as a factor in the development of lung damage following exposure to silica. [Description provided by NIOSH]
• Subjects:
  Animals Biomarkers Immune System Lung Diseases Occupational Health Respiratory Tract Diseases Safety Silicon Dioxide
• Keywords:
  Animal-studies Cellular-reactions Dose-response Immunochemistry Lung-disease Pulmonary-system-disorders Respiratory-system-disorders Silica-dusts
• ISSN:
  1528-7394
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  118-127
• Volume:
  70
• Issue:
  2
• NIOSHTIC Number:
  nn:20031616
• Citation:
  J Toxicol Environ Health A 2007 Jan; 70(2):118-127
• Contact Point Address:
  Sidney M. Morris Jr., Ph.D., Department of Molecular Genetics and Biochemistry, W1255 Biomedical Science Tower, University of Pittsburgh School of Medicine. Pittsburgh, PA 15261
• Email:
  smorris@pitt.edu
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9) ; Quartz (SiO2) (CAS RN 14808-60-7) ; Silica (CAS RN 7631-86-9)
• Federal Fiscal Year:
  2007
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Toxicology and Environmental Health, Part A: Current Issues
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:cf44c05c9c6a17073843692bb4038a513b6e789dc3d52c57f101eba7b5f372393a041e1ddb120cf5e0617f3741b136ec8170b61613d0bab7e48cf534ac7bce2d
• Download URL:
  https://stacks.cdc.gov/view/cdc/185861/cdc_185861_DS1.pdf
• File Type:
  [PDF - 360.22 KB ]