Interlaboratory Evaluation of a Portable Fluorescence Method for the Measurement of Trace Beryllium in the Workplace

Details

• Personal Author:
  Agrawal, Anoop ; Ashley, Kevin E. ; Brisson M ; Cronin J ; Goodyear G ; McCleskey T
• Description:
  Researchers at Los Alamos National Laboratory (LANL) developed a field-portable fluorescence method for the measurement of trace beryllium in workplace samples such as surface dust and air filters. The technology has been privately licensed and is commercially available. In cooperation with the Analytical Subcommittee of the Beryllium Health and Safety Committee, we have carried out a collaborative interlaboratory evaluation of the LANL field-portable fluorescence method. The interlaboratory study was conducted for the purpose of providing performance data that can be used to support standard methods. Mixed cellulose ester (MCE) membrane filters and Whatman 541 filters were spiked with beryllium standard solutions so that the filters spanned the range approximately 0.05 - 0.5 micrograms Be per sample. Sets of these filters were then coded (to ensure blind analysis) and sent to participating laboratories, where they were analyzed. Analysis consisted of the following steps: 1. removal of the filters from transport cassettes and placement of them into 15-mL centrifuge tubes; 2. mechanically-assisted extraction of the filters in 5 mL of 1% ammonium bifluoride solution (aqueous) for 30 min; 3.-4. filtration and transfer of sample extract aliquots (100 microgramsL) into fluorescence cuvettes; 5. introduction of 1.9 mL of detection solution (to effect reaction of the fluorescence reagent with beryllium in the extracted sample); and 6. measurement of fluorescence at approximately 475 nm using a portable fluorometer. This work presents performance data in support of a procedure that is targeted for publication as a National Institute for Occupational Safety and Health (NIOSH) method and as an ASTM International standard. [Description provided by NIOSH]
• Subjects:
  Aerosols Beryllium Laboratories Occupational Health Safety Workplace
• Keywords:
  Analytical-methods Beryllium-compounds Laboratory-techniques Sampling Sampling-methods Standards Work-environment
• ISBN:
  9780803134997
• Publisher:
  ASTM International
• Document Type:
  Text
• Genre:
  Book
• Place as Subject:
  Arizona ; New Mexico ; Ohio ; OSHA Region 4 ; OSHA Region 5 ; OSHA Region 6 ; OSHA Region 9 ; South Carolina
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  102-109
• NIOSHTIC Number:
  nn:20031601
• Citation:
  Beryllium: sampling and analysis. STP 1473, Ashley K, ed. West Conshohocken, PA: ASTM International, 2006 Dec; :102-109
• Contact Point Address:
  K. Ashley, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Mail Stop R-7, 4676 Columbia Parkway, Cincinnati, OH 45226
• Email:
  KAshley@cdc.gov
• CAS Registry Number:
  Beryllium (CAS RN 7440-41-7)
• Federal Fiscal Year:
  2007
• Peer Reviewed:
  False
• Source Full Name:
  Beryllium: sampling and analysis
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:92e18d109992f05daf6f312611cd1236de47b9c60a41d3beea438ae7426f34ac0ca63cc07229d778046d37cd3dc725a198ae1c66b81967b8de550919a4a553c0
• Download URL:
  https://stacks.cdc.gov/view/cdc/185847/cdc_185847_DS1.pdf
• File Type:
  [PDF - 945.36 KB ]