Detection of styrene oxide-DNA adducts by 32P-postlabeling.

Details

• Personal Author:
  Bodell WJ ; Liu S-F ; Rappaport SM ; Rasmussen J
• Description:
  The formation of DNA adducts by styrene-7,8-oxide (96093) (styrene- oxide) was studied in-vitro. One to 30 microliters (microl) of styrene-oxide were reacted with 0.5 milligram calf thymus DNA, 2'- deoxyguanosine-3'-monophosphate (dGp), 2'-deoxyadenosine-3'- monophosphate (dAp), 2'-deoxycytidine-3'-monophosphate (dCp), or 2'- deoxythymidine-3'-monophosphate (dTp) in a Tris/hydrochloric-acid buffer, pH 7.5, at 37 degrees-C for about 15 hours. The mixtures were assayed for adducts using a phosphorus-32 (P-32) postlabeling technique. Five styrene-oxide/DNA adducts were detected. The overall extent of DNA modification by 5microl styrene-oxide was approximately 4.4x10(-4) adducts per nucleotide. Styrene-oxide formed five adducts with dGp and two with dAp. Thin layer chromatography of the adducts on polyethyleneimine plates showed that the styrene-oxide/DNA adducts were similar to the corresponding DNA adducts. No adducts were detected with dCp or dTp. Additionally 9L cells were treated with 1 millimolar styrene-oxide for 24 hours. The cells were examined for DNA adducts as before. Several adducts were detected; the overall extent of DNA modification by styrene-oxide amounted to 19.5x10(-6) adducts/nucleotide. The adducts seem to be similar to those formed with calf thymus DNA. The authors conclude that guanine is the major site for DNA modification by styrene-oxide. The P-32 postlabeling technique can be used to detect formation of styrene- oxide/DNA adducts. [Description provided by NIOSH]
• Subjects:
  Epoxy Compounds Occupational Health Safety
• Keywords:
  Chemical-binding DNA-adducts Epoxides Grants-other In-vitro-studies Molecular-structure NIOSH-Grant NIOSH-Publication Nucleic-acids Organic-chemicals
• ISSN:
  0143-3334
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  9
• Issue:
  8
• NIOSHTIC Number:
  nn:00182076
• Citation:
  Carcinogenesis 1988 Aug; 9(8):1401-1404
• Contact Point Address:
  Biomedical & Environ Hlth Scis University of California School of Public Health Berkeley, CA 94720
• CAS Registry Number:
  Styrene oxide (CAS RN 96-09-3)
• Federal Fiscal Year:
  1988
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other
• Performing Organization:
  University of California Berkeley, Berkeley, California
• Peer Reviewed:
  True
• Start Date:
  19850901
• Source Full Name:
  Carcinogenesis
• End Date:
  19931227
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0b75bc37f71dccc742bdd7f86b8aa88749ea6ac4dc4ebca5c4b88e78a61496d6bd0ae03e672d89dc825488ea471ca0b42f843f7fd831e325bda70ad839795717
• Download URL:
  https://stacks.cdc.gov/view/cdc/185624/cdc_185624_DS1.pdf
• File Type:
  [PDF - 1.61 MB ]