Interlaboratory studies with the Chinese hamster V79 cell metabolic cooperation assay to detect tumor-promoting agents.

Details

• Personal Author:
  Barfknecht TR ; Bohrman JS ; Burg, Jeanne R. ; Dames, Barbara L. ; Elmore E ; Gulati DK ; Langenbach R ; Niemeier RW ; Toraason M ; Barfknecht TR ; Bohrman JS ; Burg, Jeanne R. ; Dames, Barbara L. ; Elmore E ; Gulati DK ; Langenbach R ; Niemeier RW ; Toraason M
• Description:
  Metabolic cooperation assays were conducted with Chinese-hamster-V79 cells at three separate laboratories using standardized protocols and chemicals of known tumor promoting activity. Each laboratory used identical lots of test chemicals, solvents, serum, medium, and trypsin as well as a standard protocol. The chemicals to be tested were either known tumor promoters or had related chemical structures. Among the test chemicals were phorbol esters, barbiturates, antioxidants, peroxides, bladder tumor promoters, alkanes, phenol and metabolites, and others. Agreement between laboratories was found for nine of 15 chemicals tested at three laboratories. For 23 chemicals tested in two laboratories, agreement was complete on 16 chemicals. Interlaboratory agreement appeared good when mean values for cloning efficiency for the entire study were examined, but the range of individual experiment means within a laboratory was quite large. The authors conclude that several characteristics of the assay may limit its usefulness for screening broad classes of chemicals for potential activity as tumor promoters. Many chemicals show organ specific effects and it may be unrealistic to expect any individual cell type to detect all such chemicals. [Description provided by NIOSH]
• Subjects:
  Biological Assay Carcinogens Cells, Cultured Energy Metabolism Mutagens Occupational Health Safety
• Keywords:
  Bioassays Biotransformation Carcinogenesis Cell-cultures In-vitro-studies Metabolic-study NIOSH-Author Tumorigenesis
• ISSN:
  0893-6692
• Document Type:
  Text
• Genre:
  Journal Article ; Journal Article
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Pages in Document:
  33-51
• Volume:
  12
• Issue:
  1
• NIOSHTIC Number:
  nn:00181453
• Citation:
  Environ Mol Mutagen 1988 Jul; 12(1):33-51
• Federal Fiscal Year:
  1988
• Peer Reviewed:
  True
• Source Full Name:
  Environmental and Molecular Mutagenesis
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e0d5c25452c27c22b311a7b141ffb6fc090774b7f05f81a2db3fdb72ae46be5f06ad8767fa885c556bcebe4ed12348bb256afc65eedca7f7fda8902f96763038
• Download URL:
  https://stacks.cdc.gov/view/cdc/185595/cdc_185595_DS1.pdf
• File Type:
  [PDF - 1.12 MB ]