Microbial containment in conventional fermentation processes.

Details

• Personal Author:
  Cusick LB ; Jones, James H. ; Martinez, Kenneth F. ; Sheehy, John W.
• Description:
  Engineering controls in conventional enzyme fermentation operations were evaluated to identify effective controls applicable to processes involving microorganisms. Walk through surveys were conducted at six fermentation facilities, with in/depth surveys conducted at three of the sites. The various control measures were evaluated by collecting environmental air samples for the microorganisms involved in the manufacturing processes. Bioaerosol samples were collected from potential emission sites (laboratory, seed tank, fermentor tank) using a cascade impactor; factory production strain was identified using a standardized microbiological test method combined with a sugar utilization profile. Degree of microbial containment was assessed by comparing sample concentrations around unit processes to background. Results indicated that controls were most needed around high energy operations (filtering operations, agitator shafts, sampling ports) where aerosolization was likely to occur; operator work practices contributed to the degree of microbial exposure during certain operations. The authors conclude that an effective microbial containment strategy should emphasize containment within a sealed system through engineering and appropriate work practices; monitoring and the use of personal protective equipment is also advised. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Air Pollution, Indoor Microbiology Occupational Exposure Occupational Health Safety Workplace
• Keywords:
  Air-contamination Air-quality-control Control-methods Employee-exposure NIOSH-Author Work-practices Workplace-studies
• ISSN:
  0882-8032
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Georgia ; Ohio ; OSHA Region 4 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DPSE - Division of Physical Sciences and Engineering
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  177-181
• Volume:
  3
• Issue:
  6
• NIOSHTIC Number:
  nn:00178520
• Citation:
  Appl Ind Hyg 1988 Jun; 3(6):177-181
• Contact Point Address:
  Kenneth Floyd Martinez, ANational Institute for Occupational Safety and Health, Division of Physical Sciences and Engineering, Engineering Control Technology Branch, 4676 Columbia Parkway, Cincinnati, Ohio 45226
• Federal Fiscal Year:
  1988
• Peer Reviewed:
  True
• Source Full Name:
  Applied Industrial Hygiene
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:87fd5c67a71250788563de3d8831ebc30a9063a0e3208160f63c91a92dc33b28ef1d7814cdc5617c61fa3199e43af3ae25c81f0270daaf489452eaf9e53e967b
• Download URL:
  https://stacks.cdc.gov/view/cdc/185484/cdc_185484_DS1.pdf
• File Type:
  [PDF - 707.87 KB ]