Carrageenan stimulates reduction of nitroblue tetrazolium by human neutrophils without membrane depolarization, myeloperoxidase secretion, or increased oxygen consumption.

Details

• Personal Author:
  Brestel EP ; Castranova, Vincent ; McClain EJ
• Description:
  The mode of action of carrageenan (9000071), a sulfated polyanionic polysaccharide, was investigated in human neutrophils (PMN), in a serum free medium. Carrageenan is an inflammatory agent that is commonly used to test antiinflammatory drugs. PMNs were isolated from citrated whole human blood by countercurrent elutriation and were used for in-vitro experiments, in which they were stimulated by carrageenan and tested for several parameters. Included in the criteria tested were, their effect on nitroblue-tetrazolium (NBT) reduction, oxygen consumption, hydrogen-peroxide reduction, luminol dependent chemiluminescence, release of myeloperoxidase and lactate- dehydrogenase, and membrane depolarization. Carrageenan stimulated the reduction of NBT by PMNs, but did not stimulate membrane depolarization, oxygen consumption, hydrogen-peroxide production, or myeloperoxidase secretion. The authors conclude that carrageenan stimulates a heat labile, NBT reducing system, a mechanism different from that of other PMN activators. [Description provided by NIOSH]
• Subjects:
  Blood Immune System Occupational Health Safety
• Keywords:
  Blood-cells Hemoproteins Humans Immune-reaction Immune-system In-vitro-studies Leukocytes NIOSH-Author
• ISSN:
  0360-3997
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  ALOSH - Appalachian Laboratory for Occupational Safety and Health
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  425-434
• Volume:
  10
• Issue:
  4
• NIOSHTIC Number:
  nn:00167931
• Citation:
  Inflammation 1986 Dec; 10(4):425-434
• CAS Registry Number:
  Carrageenan (CAS RN 9000-07-1)
• Federal Fiscal Year:
  1987
• Peer Reviewed:
  True
• Source Full Name:
  Inflammation
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:dc83daf5cef9d6569f746e8ec730a6daf0eb7b7799e69e6bb27aaef040bbe529e42d9ce426910e70ee8d6f9e16e887df1cf3bd2ba59301651dd978c53e0a1917
• Download URL:
  https://stacks.cdc.gov/view/cdc/185150/cdc_185150_DS1.pdf
• File Type:
  [PDF - 498.57 KB ]