Analyses of ethylene glycol monoalkyl ethers and their proposed metabolites in blood and urine.

Details

• Personal Author:
  DeBord KE ; Lowry, Larry K. ; Smallwood, Anthony W.
• Description:
  Techniques were developed for the analysis of ethylene glycol monoalkyl ethers and their metabolites in human blood and urine. Procedures for measuring 2-methoxyethanol (109864), 2-ethoxyethanol (110805) and 2-butoxyethanol (111762) in blood or their oxidized acidic metabolites methoxyacetic-acid (625456) and ethoxyacetic-acid (627032) in urine were designed to permit evaluation of worker uptake of these solvents. A stock solution of the glycol ethers was mixed with pooled human blood to obtain concentrations from 8 to 1000 micrograms per gram (microg/g). The blood was mixed with methylene-chloride for 2.5 hours, filtered and the methylene- chloride extract reduced in volume for injection into a gas chromatograph with flame ionization detector using a stainless steel column packed with Carbowax on mesh. A stock solution of pooled urine containing the metabolites at concentrations from 10 to 1000microg/milliliter (ml) was acidified with hydrochloric-acid and extracted with methylene-chloride three times. The extracts were evaporated to 1ml and derivatized with a pentafluorobenzylation procedure before injection into the gas chromatograph. Approximate retention times for 2-methoxyethanol, 2-ethoxyethanol and 2- butoxyethanol were 5.0, 5.4, and 8.0 minutes respectively. Detection limits for 2-ethoxyethanol and 2-butoxyethanol were 5.0 and 4.0microg/g blood, while 2-methoxyethanol had a detection limit of 8.8microg/g. Average recovery data ranged from 78 to 84 percent over a large concentration range. Approximate retention times for methoxyacetic-acid and ethoxyacetic-acid in urine were 4.9 and 5.4 minutes each. Detection limits were 11.4 and 5.0microg/ml. Recoveries over the concentration range studied were 78 percent for methoxyacetic-acid and 91 percent for ethoxyacetic-acid. The authors conclude that these techniques are ready for application in biological monitoring of workers exposed to these solvents. [Description provided by NIOSH]
• Subjects:
  Biochemistry Biology Blood Hematologic Tests Occupational Exposure Occupational Health Safety Urine
• Keywords:
  Analytical-methods Analytical-processes Biochemical-analysis Biochemical-tests Biological-effects Biological-monitoring Blood-analysis Blood-cells NIOSH-Author Occupational-exposure Urinalysis

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• ISSN:
  0091-6765
• Document Type:
  Text
• Genre:
  Journal Article
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  249-253
• Volume:
  57
• NIOSHTIC Number:
  nn:00144031
• Citation:
  Environ Health Perspect 1984 Aug; 57:249-253
• CAS Registry Number:
  2-Butoxyethanol (CAS RN 111-76-2) ; 2-Methoxyethanol (CAS RN 109-86-4) ; Ethoxyacetic acid (CAS RN 627-03-2) ; Glycol monoethyl ether (CAS RN 110-80-5) ; Methoxyacetic acid (CAS RN 625-45-6)
• Federal Fiscal Year:
  1984
• Peer Reviewed:
  True
• Source Full Name:
  Environmental Health Perspectives
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f8459bec4883c8439dfe57ce1f9e21557a3cf0efdcd7698f50b59243ba3cc85f14db95d4fa307f17d85e5de11c36f97aef53c4d552f88778071ee31f2bcaf8c6
• Download URL:
  https://stacks.cdc.gov/view/cdc/184795/cdc_184795_DS1.pdf
• File Type:
  [PDF - 761.00 KB ]