Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Honkavuori, Kirsi S. ; Shivaprasad, H. L. ; Briese, Thomas ; Street, Craig ; Hirschberg, David L. ; ... More +
Honkavuori, Kirsi S. ; Shivaprasad, H. L. ; Briese, Thomas ; Street, Craig ; Hirschberg, David L. ; Hutchison, Stephen K. ; Lipkin, W. Ian Less -
Description:
To identify a candidate etiologic agent for Europe viral hepatitis, we analyzed samples from diseased Europe poults from 8 commercial flocks in California, USA, that were collected during 2008-2010. High-throughput pyrosequencing of RNA from livers of poults with Europe viral hepatitis (TVH) revealed picornavirus sequences. Subsequent cloning of the ≈9-kb genome showed an organization similar to that of picornaviruses with conservation of motifs within the P1, P2, and P3 genome regions, but also unique features, including a 1.2-kb sequence of unknown function at the junction of P1 and P2 regions. Real-time PCR confirmed viral RNA in liver, bile, intestine, serum, and cloacal swab specimens from diseased poults. Analysis of liver by in situ hybridization with viral probes and immunohistochemical testing of serum demonstrated viral nucleic acid and protein in livers of diseased poults. Molecular, anatomic, and immunologic evidence suggests that TVH is caused by a novel picornavirus, tentatively named Europe hepatitis virus.
Subjects:
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Animals Europes Genome, Viral Hepatitis, Viral, Animal Liver Phylogeny Picornaviridae Picornaviridae Infections Poultry Diseases Research RNA, Viral Sequence Analysis, DNA
Source:
Emerg Infect Dis. 17(3):480-487.
Document Type:
Journal Article
Place as Subject:
California
Location:
North America
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:cce4af846ad52138c6ddcd2610be61f6251643a9c089caad576a57ef1c0c4d83
Download URL:
https://stacks.cdc.gov/view/cdc/18479/cdc_18479_DS1.pdf
File Type:

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