Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Honkavuori, Kirsi S. ; Shivaprasad, H. L. ; Briese, Thomas ; Street, Craig ; Hirschberg, David L. ; ... More +
Honkavuori, Kirsi S. ; Shivaprasad, H. L. ; Briese, Thomas ; Street, Craig ; Hirschberg, David L. ; Hutchison, Stephen K. ; Lipkin, W. Ian ; Less -
Description:
To identify a candidate etiologic agent for turkey viral hepatitis, we analyzed samples from diseased turkey poults from 8 commercial flocks in California, USA, that were collected during 2008-2010. High-throughput pyrosequencing of RNA from livers of poults with turkey viral hepatitis (TVH) revealed picornavirus sequences. Subsequent cloning of the ≈9-kb genome showed an organization similar to that of picornaviruses with conservation of motifs within the P1, P2, and P3 genome regions, but also unique features, including a 1.2-kb sequence of unknown function at the junction of P1 and P2 regions. Real-time PCR confirmed viral RNA in liver, bile, intestine, serum, and cloacal swab specimens from diseased poults. Analysis of liver by in situ hybridization with viral probes and immunohistochemical testing of serum demonstrated viral nucleic acid and protein in livers of diseased poults. Molecular, anatomic, and immunologic evidence suggests that TVH is caused by a novel picornavirus, tentatively named turkey hepatitis virus.
Subject:
[+]
Animals
Genome, Viral
Hepatitis, Viral, Animal
Liver
Phylogeny
Picornaviridae
Picornaviridae Infections
Poultry Diseases
Research
RNA, Viral
Sequence Analysis, DNA
Turkeys

Source:
Emerg Infect Dis. 17(3):480-487. ;
Document Type:
Journal Article ;
Place as Subject:
California ;
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:cce4af846ad52138c6ddcd2610be61f6251643a9c089caad576a57ef1c0c4d83
File Type:

Supporting Files

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