Chemical characterization of isocyanate-protein conjugates.

Details

• Personal Author:
  Pesce AJ ; Tse CS
• Description:
  The rates of reaction of toluene-diisocyanate (26471625) (TDI) and hexamethylene-diisocyanate (822060) (HDI) with human serum albumin (HSA) were studied. Reactions of HDI and TDI with HSA were carried out at room temperature. An 0.9 gram (g) of HSA in solution was reacted with 2.1g or 0.21g HDI or 2.4g TDI. Aliquots were taken at selected time intervals after the start of the reaction. Ammonium- carbonate was used to stop the reactions, and portions of the isocyanate protein conjugates were purified by centrifugation, dialysis, and precipitation. Assays were performed for the quantity of isocyanate bound to protein, protein content, amino acid residues, and free amino groups. Protein derivatives from the reaction of TDI with HSA consisted of 78 percent bisureido and 22 percent monoureido forms. TDI bound protein reached a maximum at 20 minutes. The number of recovered lysine residues was the same in unconjugated HSA as in TDI HSA conjugates. In both high and low concentrations of HDI, the amount of isocyanate bound to protein as the amine hexamethylenediamine increased with time. The only significant change in amino acid residues recovered from HSA before and after reaction with HDI occurred in the lysyl residues. At 0 minutes, 47.36 nanomoles (nmol) were recovered; after 180 minutes, 22.60nmol were recovered. The authors conclude that reaction time is the most important factor in protein conjugation with aromatic and aliphatic hydrocarbons. [Description provided by NIOSH]
• Subjects:
  Amino Acids Biochemistry Chemistry Fatty Acids Isocyanates Occupational Health Polycyclic Aromatic Hydrocarbons Safety
• Keywords:
  Aliphatic-compounds Amino-acids Aromatic-hydrocarbons Biochemical-analysis Chemical-composition Molecular-biology NIOSH-Grant NIOSH-Publication Protein-biosynthesis Reaction-rates
• ISSN:
  0041-008X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  39-46
• Volume:
  51
• Issue:
  1
• NIOSHTIC Number:
  nn:00138644
• Citation:
  Toxicol Appl Pharmacol 1979 Oct; 51(1):39-46
• Contact Point Address:
  Internal Medicine University of Cincinnati 231 Bethesda Avenue Cincinnati, Ohio 45267
• CAS Registry Number:
  Hexamethylene diisocyanate (CAS RN 822-06-0) ; Toluene diisocyanate (CAS RN 26471-62-5)
• Federal Fiscal Year:
  1980
• Performing Organization:
  University of Cincinnati, Cincinnati, Ohio
• Peer Reviewed:
  True
• Start Date:
  19780701
• Source Full Name:
  Toxicology and Applied Pharmacology
• End Date:
  19810630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c08ba75adb997e2e12e207dc9e511f185edf4f42c02bf740760fd59ed61c172fbf87e0026d6544a30335cd89fcc13aa43e82b8af077f62b729d9f4497aeac8d2
• Download URL:
  https://stacks.cdc.gov/view/cdc/184720/cdc_184720_DS1.pdf
• File Type:
  [PDF - 692.23 KB ]