In vivo cytogenetic studies on mice exposed to ethylene dibromide.

Details

• Personal Author:
  Krishna G ; Nath J ; Ong T ; Petersen, Martin R. ; Xu J
• Description:
  The genotoxicity of ethylene-dibromide (106934) (EDB) was evaluated using in-vivo cytogenetic assays. EDB was injected intraperitoneally (ip) into male CD-1-mice. Based on median lethal doses, EDB concentrations of 42, 84, and 168 milligrams per kilogram (mg/kg) were selected for cytogenetic studies. Bone marrow cells isolated from femora were analyzed for sister chromatid exchange (SCE), chromosomal aberration, and micronucleus formation. The frequencies of the first, second, and third generation metaphases were determined in 100 consecutive metaphase cells from each animal for evaluating cellular proliferation. The incidence of micronucleated cells per 500 polychromatic erythrocytes (PCE) was determined in each animal. The ratio of normochromatic erythrocytes (NCE) to PCE was also calculated to determine the toxic effects of EDB to bone marrow cells. Among the three doses tested, 84 and 168mg/kg EDB yielded 6.09 and 5.47 SCEs per cell, respectively. The number of first, second and third, or subsequent division cells were in close approximation for all treatments and in controls. Following in-vivo exposure to EDB, the group exposed to 42mg/kg had a slight but significant increase in chromosomal aberrations over the negative control group; aberrations consisted of gaps, breaks, and fragments. The number of PCE with micronuclei was not significantly different between the controls and treated cells. The ratio of NCE to PCE was similar between the control and treated animals. The authors conclude that EDB is not an effective genotoxic agent in-vivo in mice. [Description provided by NIOSH]
• Subjects:
  Chromosomes Ethylenes Genetics Hazardous Substances Inhalation Exposure Laboratories Musculoskeletal System Occupational Health Safety
• Keywords:
  Bone-marrow Cell-metabolism Chromosome-disorders Genes Humans In-vivo-studies Inhalants Laboratory-animals Lethal-dose NIOSH-Author Toxic-effects

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• ISSN:
  0027-5107
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  81-87
• Volume:
  158
• Issue:
  1
• NIOSHTIC Number:
  nn:00134920
• Citation:
  Mutat Res 1985 Oct-Nov; 158(1-2):81-87
• CAS Registry Number:
  1,2-Dibromoethane (CAS RN 106-93-4)
• Federal Fiscal Year:
  1986
• Peer Reviewed:
  True
• Source Full Name:
  Mutation Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6012fbdac18a14558a790c8ce0bb7f4767ad1c9e9b1e5c6f96f725f8a6f5faef0acc45b8b1fc10de6d6eff6e214528d49b54477d34ce9bdb445e2245ea723a40
• Download URL:
  https://stacks.cdc.gov/view/cdc/184671/cdc_184671_DS1.pdf
• File Type:
  [PDF - 504.95 KB ]