Conversion of C3 and consumption of hemolytic complement by extracts of airborne grain dust.

Details

• Personal Author:
  Major, Pervis C. ; Mull JC ; Olenchock, Stephen A.
• Description:
  The effects of aqueous extractions of airborne grain dust on human C3 and hemolytic complement were studied. The involvement of the calcium independent alternative complement pathway in C3 conversion was investigated by differential chelation of magnesium and calcium with ethylenediamine-tetracetic-acid (60004) (EDTA) or ethyleneglycol-bis-(beta-amino-ethyl-ether)-N,N'-tetraacetic-acid (67425) (EGTA). Airborne oat and spring wheat dusts were obtained and dust extracts were mixed with normal human serum. The resulting hemolytic complement and functional C3 concentrations were compared with untreated normal human serum. Increasing amounts of dust extracts caused increasing conversion of C3 to C3b. Spring wheat dust extract was much more toxic to hemolytic complement activity than the extract of oat dust. Differential chelation revealed that serum with only chelator calcium or C1 did not convert C3 to C3b. Sera pretreated with EDTA and reacted with grain extracts blocked C3 conversion whereas those serums treated with EGTA demonstrated arc conversion from C3 to C3b. The authors suggest that the alternative complement pathway is involved in C3 conversion by grain dust extracts. [Description provided by NIOSH]
• Subjects:
  Biochemistry Chelating Agents Dust Hematologic Tests Occupational Health Safety
• Keywords:
  Biochemical-analysis Blood-serum Chelates Hematology Humans NIOSH-Author Plant-dusts
• ISSN:
  0012-3692
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  225-227
• Volume:
  75
• Issue:
  2
• NIOSHTIC Number:
  nn:00117486
• Citation:
  Chest 1979 Feb; 75(2):225-227
• CAS Registry Number:
  EGTA (CAS RN 67-42-5) ; Ethylenediaminetetraacetic acid (CAS RN 60-00-4)
• Federal Fiscal Year:
  1979
• Peer Reviewed:
  True
• Source Full Name:
  Chest
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:face49cc92137ce207516395003ce92c0fbf648df46d4971da74349bde59960357f9ab0cdde04fa48d4a63397a536f83dc5ef144c117304087e5e7496cbe85b2
• Download URL:
  https://stacks.cdc.gov/view/cdc/184467/cdc_184467_DS1.pdf
• File Type:
  [PDF - 376.39 KB ]