Biomedical applications of backscattered electron imaging: one year's experience with SEM histochemistry.

Details

• Personal Author:
  Abraham JL ; DeNee PB
• Description:
  The usefulness of backscattered electron (BSE) imaging in scanning electron microscopic (SEM) study of biological samples was evaluated. Tissue samples were mounted on supports of low average atomic number, critical point dried using carbon-dioxide, and coated with carbon. An image resembling a light microscopic or transmission electron microscopic image was obtained with the BSE mode using reversed signal polarity. BSE mode resolution and SEM depth of field greatly exceeded that of microscopy. The BSE image revealed stained features several micrometers beneath the specimen surface because penetration was greater than with the secondary electron mode. The BSE mode revealed vascular patterns not seen in the secondary electron mode in unstained lungs with pneumoconioses, and also showed the location of retained dust particles. Nuclei, nucleoli, reticulin fibers, basement membranes, lysosomes and enzyme activities also were easily identified in other tissue sections and blocks, using modifications of existing stains. The fine structural surface morphology was not altered by treatment of tissues with histochemical reagents after fixation or by critical point drying. The authors conclude that BSE imaging will become at least as valuable in SEM as fluorescence and polarizing microscopy are for light microscopy. [Description provided by NIOSH]
• Subjects:
  Laboratories Lung Occupational Health Pathology Respiratory System Safety
• Keywords:
  Analytical Instruments Analytical Methods Analytical Processes Atomic Number Contrast Author Keywords: Scanning Electron Microscope Backscattered Electrons Biological Material Enzyme Histochemistry Glomerulonephritis Heavy Metal Stains Kidney Laboratory Techniques Lungs Microscopy Pneumoconiosis Resolution

  More +
• ISBN:
  9780070692534
• Publisher:
  McGraw-Hill
• Document Type:
  Text
• Genre:
  Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  ALFORD - Appalachian Laboratory for Occupational Respiratory Disease
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  251-258
• NIOSHTIC Number:
  nn:00115410
• Citation:
  Scanning electron microscopy. Wells OC, ed. New York: McGraw-Hill, :251-258
• Editor(s):
  Wells OC
• Federal Fiscal Year:
  1974
• Peer Reviewed:
  False
• Part Number:
  1
• Source Full Name:
  Scanning electron microscopy: Proceedings of the Seventh Annual, Scanning Electron Microscope Symposium, Chicago, Illinois, April 1974
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:642eff6c566bb21b7574d80b71a53f2269a5862d1320f85ae4ccdf60c4e8733f3bd2856c64034b817ce69b500c0fda45df7afeb94edd30ba98cdebd68dad2ad9
• Download URL:
  https://stacks.cdc.gov/view/cdc/184438/cdc_184438_DS1.pdf
• File Type:
  [PDF - 4.44 MB ]