Trace analysis of potentially carcinogenic metabolites of an azo dye and pigment in hamster and human urine as determined by two chromatographic procedures.

Details

• Personal Author:
  Bowman MC ; Nony CR
• Description:
  Electron capture/gas chromatography (EC/GC) and high pressure liquid chromatography (HPLC) were used to detect the metabolites of Direct Black 38 (1937377) and Pigment Yellow 12 (6358856) in hamster and human urine. Analytic procedures included: extraction of the free aromatic amines and neutral compounds; alkaline hydrolysis of the aqueous phase and extraction of any hydrolyzed conjugates as free amines; and analysis of the free amines and acetylated metabolites directly by high pressure liquid chromatography or electron capture gas chromatography after conversion of the amines to heptafluorobutyryl derivatives. Metabolites in hamster and human urine were detected at concentrations as low as 1 part per billion. The authors recommend this approach for monitoring exposed workers since it will also detect any other derivatizable aromatic amines. [Description provided by NIOSH]
• Subjects:
  Carcinogens Chemistry Coloring Agents Energy Metabolism Manufacturing And Industrial Facilities Occupational Health Safety Urine Workplace
• Keywords:
  Analytical-methods Chemical-analysis Coloring-materials Contract-224-78-0004 Dyestuffs Industrial-factory-workers Metabolic-effects Monitoring-system NIOSH-Contract NIOSH-Publication Urinalysis Work-environment

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• ISSN:
  0021-9665
• Document Type:
  Text
• Funding:
  Contract
• Genre:
  Journal Article
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  64-74
• Volume:
  18
• Issue:
  2
• NIOSHTIC Number:
  nn:00094932
• Citation:
  J Chromatogr Sci 1980 Feb; 18(2):64-74
• CAS Registry Number:
  Direct Black 38 (CAS RN 1937-37-7) ; Pigment Yellow 12 (CAS RN 6358-85-6)
• Federal Fiscal Year:
  1980
• Peer Reviewed:
  False
• Source Full Name:
  Journal of Chromatographic Science
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d89f7a1e2b1ef6c5ab56273395b9c02a81d9ce41dfcace78864143766ad6cb1478955fd3ba8d14fe72816b6c70688e22116fe12ef7f47db39fcd61c9465cba75
• Download URL:
  https://stacks.cdc.gov/view/cdc/184343/cdc_184343_DS1.pdf
• File Type:
  [PDF - 1.69 MB ]