Induction of 6-thioguanine resistance in Chinese hamster lung cells treated with dimethylnitrosamine' 2-amino-anthracene or 7,12-dimethylbenz(A)anthracene in the presence of rat liver microsomes.

Details

• Personal Author:
  Huang SL ; Whong, Wen-Zong
• Description:
  The mutagenic effects of dimethylnitrosamine (62759) (DMN), 2- aminoanthracene (613138) (2-AA), and 7,12-dimethylbenz(a)anthracene (57976) (7,12-DMBA) on cultured Chinese hamster lung cells were investigated at the hypoxanthine guanine phosphoribosyl transferase (HGPRT) locus. All three compounds induce 6-thioguanine (6TG) resistance only in the presence of rat liver microsomes. The mutation frequencies induced by the compounds are dose dependent. The authors conclude that DMN, 2-AA, and 7,12-DMBA require metabolic activation for mutagenicity. They suggest that the mutagenic potential of carcinogens and other chemical agents can be detected and quantitated through the use of Chinese hamster cells and microsome activation. [Description provided by NIOSH]
• Subjects:
  Diagnosis Environmental Exposure Enzymes Laboratories Mutagens Occupational Exposure Occupational Health Oxidants Safety
• Keywords:
  Analytical-methods Cellular-reactions Chemical-exposure Diagnostic-techniques Enzymatic-activity Laboratory-animals Mutagenesis NIOSH-Author Oxidative-metabolism Subcellular-structure
• ISSN:
  0378-4274
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  209-214
• Volume:
  3
• Issue:
  4
• NIOSHTIC Number:
  nn:00093839
• Citation:
  Toxicol Lett 1979 Apr; 3(4):209-214
• CAS Registry Number:
  2-Aminoanthracene (CAS RN 613-13-8) ; 7,12-Dimethylbenz[a]anthracene (CAS RN 57-97-6) ; N-Nitrosodimethylamine (CAS RN 62-75-9)
• Federal Fiscal Year:
  1979
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology Letters
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e6c41da8ee66fc89ab094091bf5b237059cdefcf1c1dd6a63ead1a11067be48bed74a86db2fcbcfc2490675918e87981a164a5c72a8bc8d97d0733e37d96a075
• Download URL:
  https://stacks.cdc.gov/view/cdc/184337/cdc_184337_DS1.pdf
• File Type:
  [PDF - 373.81 KB ]