Comparison of scanning transmission and backscattered electron imaging in the SEM, using heavy metal stained sections of biological tissue.

Details

• Personal Author:
  DeNee PB ; Frederickson RG
• Description:
  The results of a study which compared the scanning transmission electron microscope (STEM) and the backscattered electron (BSD) imaging in the scanning electron microscope (SEM) using embryonic chick tissue stained with uranyl-acetate (541093) and lead-citrate (14450603) are reported. It was determined that STEM was marginally adequate for sections of 60nm to 100nm and optimal at 200nm; however, above 200nm, resolution deteriorated and was uninformative. The BSE image was poor for sections of 60nm to 100nm and became optimal for thicknesses and 200nm and greater. [Description provided by NIOSH]
• Subjects:
  Laboratories Occupational Health Safety
• Keywords:
  Analytical-instruments Analytical-methods Laboratory-equipment
• Publisher:
  Claitor's Publishing
• Document Type:
  Text
• Genre:
  Proceedings
• Place as Subject:
  Florida ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  ALOSH - Appalachian Laboratory for Occupational Safety and Health
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-2
• NIOSHTIC Number:
  nn:00082643
• Citation:
  34th Annual Proceedings Electron Microscopy Society of America, Miami Beach, Florida, Bailey GW, ed. Baton Rouge, LA: Claitor's Publishing, 1976 Jan; :1-2
• CAS Registry Number:
  1,2,3-Propanetricarboxylic acid, 2-hydroxy-, lead salt (1:?) (CAS RN 14450-60-3) ; Uranyl acetate (CAS RN 541-09-3)
• Editor(s):
  Bailey GW
• Federal Fiscal Year:
  1976
• Peer Reviewed:
  False
• Source Full Name:
  34th Annual Proceedings Electron Microscopy Society of America, Miami Beach, Florida,
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:26ccbfeddb1b436e9fe47c264344810e41404b18b58a813c0ad7398a60ce43046cc663c8845c867587a4f3addd5d5ef2883d28cde996e61b42364ad11392927a
• Download URL:
  https://stacks.cdc.gov/view/cdc/184257/cdc_184257_DS1.pdf
• File Type:
  [PDF - 865.36 KB ]