Cell attachment and penetration by influenza virus.

Details

• Personal Author:
  Booth JA ; Eckert HL ; Hahon N
• Description:
  The attachment and penetration of cells by influenza viruses and the environmental factors affecting the efficiency of these interactions are reported. Attachment and penetraon of influenza virus into clone 1-5C-4 cells are quantitatively determined by the immunofluorescent cell-counting assay. Aided by centrifugal force, more than 95% of virus inocula of five representative influenza virus strains are attached to cells at a linear rate within 10 minutes, in contrast to approximately 35% after stationary incubation at 35 degrees centigrade for 2 hours. By the former procedure, a proportionality between the number of infected cells and volume of inoculum is revealed which is not evident when stationary incubation is employed. Maximal binding of virus to cells occurs at 0.2 sodium chloride. The salt requirement, added to evidence of pH dependence and temperature inependence, indicates that the initial virus-cell union involves electrostatic forces. Virus penetration into cells, measured by the insensitivity of virus-cell complexes to antiviral serum, is linear and complete within 15 minutes at 35 degrees centigrade for all five virus strains tested. Maximal virus penetration occurs at 0.1 to 0.2 molar, the process is pH- and temperature-dependent. Both virus attachment and penetration processes are partially inhibited in the presence of diethylaminoethyl-dextran. [Description provided by NIOSH]
• Subjects:
  Communicable Diseases Occupational Health Safety
• Keywords:
  Absorption Biochemical Reactions Cell Membrane Chemical Binding Communicable Diseases Electrochemical Properties Infectious Diseases Viruses
• ISSN:
  0019-9567
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  ALFORD - Appalachian Laboratory for Occupational Respiratory Disease
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  341-351
• Volume:
  7
• Issue:
  3
• NIOSHTIC Number:
  nn:00058633
• Citation:
  Infect Immun 1973 Mar; 7(3):341-351
• CAS Registry Number:
  Silver chloride (CAS RN 7783-90-6)
• Federal Fiscal Year:
  1973
• Peer Reviewed:
  True
• Source Full Name:
  Infection and Immunity
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:19469e51918fe0249f1e15432dccf3bcdbcf27751fbc3434309d0ce0802fdc3cdc46d885be16974dcd9abe574bf909edd3665645d08a792512c40d71d8b2a368
• Download URL:
  https://stacks.cdc.gov/view/cdc/184119/cdc_184119_DS1.pdf
• File Type:
  [PDF - 1.65 MB ]