Hemadsorption cell-counting assay of interferon.

Details

• Personal Author:
  Booth JA ; Hahon N
• Description:
  A simple assay for interferon was developed that is based on the enumeration of individual cells manifesting hemadsorption within 20 to 24 hours of infection. Two assays, that of quantitative hemadsorption and immunofluorescent cell-counting were combined to develop the hemadsorption cell-counting assay of interferon described in this report. Results of a comparison in sensitivity between the hemadsorption and immunofluorescent cell-counting assays of the same interferon preparation are shown. Ten replicate determinations of the same interferon preparation were made to estimate the precision of the assay. The standard deviation was plus or minus 29.2; the coefficient of variation was 6.6%; titers ranged from 400 to 470 units/ml with a mean of 439 units. The simplicity, rapidity, high precision, and reproducibility of this procedure adequately fulfilled the proposed criteria for interferon assessment. [Description provided by NIOSH]
• Subjects:
  Anti-Bacterial Agents Antibodies Blood Cytology Hematologic Tests Immune System Occupational Health Safety
• Keywords:
  Analysis Antibiotics Antivirus Agents Hematology Immunology Virology
• ISSN:
  0003-9012
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  ALFORD - Appalachian Laboratory for Occupational Respiratory Disease
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  160-163
• Volume:
  44
• Issue:
  2
• NIOSHTIC Number:
  nn:00040968
• Citation:
  Arch Gesamte Virusforsch, 1974 Jun; 44(2):160-163
• CAS Registry Number:
  Interferons (CAS RN 9008-11-1)
• Federal Fiscal Year:
  1974
• Peer Reviewed:
  True
• Source Full Name:
  Archiv fur die Gesamte Virusforschung
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:888b2bce3ff469e2c19c179811cd948b32d33b60d568f9c8c7b415cbb7658e1e4e4e4b883fbec8da03fe4c195237601a62f37aad3515e185cb98146d27b0aef6
• Download URL:
  https://stacks.cdc.gov/view/cdc/184011/cdc_184011_DS1.pdf
• File Type:
  [PDF - 211.24 KB ]