Atomic absorption determination of zinc, copper, cadmium, and lead in tissues solubilized by aqueous tetramethylammonium hydroxide.

Details

• Personal Author:
  Eller, Peter M. ; Menden EE ; Murthy L ; Petering HG
• Description:
  Rat liver and kidney homogenates and homogeneous rat hair samples are subjected to atomic absorption spectrophotometric analysis after digestion with an appropriate concentration of aqueous tetramethylammonium hydroxide. The endogenous tissue levels of Zn, Cu, Cd and Pb, the reproducibility of the analyses, and the recovery of added metal standards compare favorably with the results obtained by standard wet ashing procedures using concentrated nitric acid or nitric acid and perchloric acids. The tetramethylammonium digestion method allows faster and safer processing and handling of samples in comparison to acid digestion procedures. [Description provided by NIOSH]
• Subjects:
  Biochemistry Metals Metals, Heavy Occupational Health Safety
• Keywords:
  Analytical-methods Biochemical-analysis Heavy-metals Histochemistry NIOSH-Grant NIOSH-Publication Sample-preparation Trace-metals
• ISSN:
  0003-2697
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  365-372
• Volume:
  53
• Issue:
  2
• NIOSHTIC Number:
  nn:00028837
• Citation:
  Anal Biochem 1973 Jun; 53(2):365-372
• Contact Point Address:
  Environmental Health Kettering Laboratory Eden & Bethesda Avenues Cincinnati, Ohio 45219
• CAS Registry Number:
  Cadmium (CAS RN 7440-43-9) ; Copper (CAS RN 7440-50-8) ; Lead (CAS RN 7439-92-1) ; Nitric acid (CAS RN 7697-37-2) ; Perchloric acid (CAS RN 7601-90-3) ; Tetramethylammonium hydroxide (CAS RN 75-59-2) ; Zinc (CAS RN 7440-66-6)
• Federal Fiscal Year:
  1973
• Performing Organization:
  University of Cincinnati, Cincinnati, Ohio
• Peer Reviewed:
  True
• Start Date:
  19710401
• Source Full Name:
  Analytical Biochemistry
• End Date:
  19730331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8c90611679d51bd43e98e9d2857d8212ed2c6d4651606f1ed7a458abb2cbdfc365cf2ad7132234e674a0cc3077a04b0b75ac1d723bacfd5d9a23f578e5fc3819
• Download URL:
  https://stacks.cdc.gov/view/cdc/183931/cdc_183931_DS1.pdf
• File Type:
  [PDF - 446.59 KB ]