Methods for a SEM study of coal workers' pneumoconiosis.

Details

• Personal Author:
  Abraham JL ; DeNee PB ; Gelderman AH
• Description:
  This Scanning Electron Microscopy (SEM) study of lung tissue obtained from the National Coal Workers' Autopsy Study presents the first SEM demonstration of respirable dust in situ in the human lung. Several methods have been developed to yield valuable morphological information in the SEM from routine formalin-fixed, paraffin-embedded tissues. Correlation of the SEM image and the light microscopic (LM) images was made. Three cases selected to illustrate the methods represent a range of severity of coal workers' pneumoconiosis (CWP): (1) focal emphysema in bituminous coal miner, (2) reticular nodular lesions in a bituminous coal miner, (3) collagenous nodular lesions in a anthracite miner. There are no clearly demonstrable qualitative differences in the silica to silicate ratios among the three cases examined. However, there is a significant difference, by source excited X-ray fluorescence, in the elemental composition of the dust in Case No. 3 compared to Case No. 2. [Description provided by NIOSH]
• Subjects:
  Coal Coal Mining Dust Fibrosis Mining Occupational Health Pathology Radiography Safety Silicon Dioxide
• Keywords:
  Author Keywords: Coal Cell-structure Coal-miners Collagen Collagens Emphysema Histochemistry Microscopy Pneumoconiosis Reticulin Scanning Electron Microscopy Silica X-ray-fluorescence-analysis

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• Publisher:
  IIT Research Institute
• Document Type:
  Text
• Genre:
  Proceedings
• Place as Subject:
  Illinois ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  ALFORD - Appalachian Laboratory for Occupational Respiratory Disease
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  411-418
• NIOSHTIC Number:
  nn:00020486
• Citation:
  Scanning Electron Microscopy, Proceedings of Workshop on Scanning Electron Microscopy in Pathology, IIT Research Institute, Chicago, Illinois, 1973 Apr; (Pt III):411-418
• CAS Registry Number:
  Silica (CAS RN 7631-86-9)
• Federal Fiscal Year:
  1973
• Peer Reviewed:
  False
• Part Number:
  III
• Source Full Name:
  Scanning Electron Microscopy, Proceedings of Workshop on Scanning Electron Microscopy in Pathology, IIT Research Institute, Chicago, Illinois
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:973537f243e9a4a370ce84b36d2bdd695062ed3b50c3470857c456bfeb140bdd7c501a718accda28ad8bc304ab5afe931d7af75abd9948a5e29ef25bea20882c
• Download URL:
  https://stacks.cdc.gov/view/cdc/183835/cdc_183835_DS1.pdf
• File Type:
  [PDF - 4.22 MB ]