Effect of quartz dust DQ 12 on human monocytes/macrophages in vitro - an electron microscopical study.

Details

• Personal Author:
  Behrendt H ; Seemayer NH
• Description:
  An investigation was provided of the surface alterations in human monocytes/macrophages in-vitro induced by quartz (14808607) DQ 12 to explain the considerably higher resistance of human macrophages to quartz particles in-vitro. At concentrations which are highly cytotoxic to guinea-pig macrophages, human monocytes/macrophages reacted with a considerably higher survival rate. Macrophages which had been exposed to 100 micrograms/milliliter of quartz DQ 12 for 48 hours displayed a vacuolar network filled with flocculant material and quartz particles but no signs of cytotoxicity. Results obtained by scanning electron microscopy and by transmission electron microscopy indicated that cultured human monocytes/macrophages displayed unique features on contact with toxic quartz particles. The higher resistance of the cells to quartz concentrations which are toxic for animal cells was underlined by a special mechanism of phagocytosis in combination with autophagolysosomal processes and cell secretion. These effects were species specific characteristics of human cultured monocytes/macrophages which have been exposed to quartz particles.
• Subjects:
  Accidents, Occupational Manufacturing And Industrial Facilities Occupational Health Safety
• Keywords:
  Airborne Dusts Cell Cultures Cytotoxic Effects Dust Exposure Dust Inhalation In Vitro Study Lung Cells Respiratory System Disorders
• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Text
• Genre:
  Conference/Symposia Proceedings
• Place as Subject:
  OSHA Region 3 ; Pennsylvania
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1459-1465
• NIOSHTIC Number:
  nn:00198709
• Citation:
  Proceedings of the VIIth International Pneumoconioses Conference, August 23-26, 1988, Pittsburgh, Pennsylvania, USA. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Institute for Occupational Safety and Health, DHHS (NIOSH) Publication No. 90-108, 1990 Nov; (Part II):1459-1465
• CAS Registry Number:
  Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  1991
• Peer Reviewed:
  False
• Part Number:
  II
• Source Full Name:
  Proceedings of the VIIth International Pneumoconioses Conference, August 23-26, 1988, Pittsburgh, Pennsylvania, USA
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3b1faade47bdad7ece2af3c0bc53ee2c1f559f9c6288de76d8e7a979a34b52c8e535080c8b8ee2e61e44759123457b459f8ee405b46f3afc812b04ad33a68d0f
• Download URL:
  https://stacks.cdc.gov/view/cdc/180622/cdc_180622_DS1.pdf
• File Type:
  [PDF - 248.21 KB ]