Top-down mass spectrometry of native proteoforms and their complexes: A community study

Details

• Alternative Title:
  Nat Methods
• Personal Author:
  Habeck, Tanja ; Brown, Kyle A. ; Soye, Benjamin Des ; Lantz, Carter ; Zhou, Mowei ; Alam, Novera ; Hossain, Md Amin ; Jung, Wonhyeuk ; Keener, James E. ; Volny, Michael ; Wilson, Jesse W. ; Ying, Yujia ; Agar, Jeffrey N. ; Danis, Paul O. ; Ge, Ying ; Kelleher, Neil L. ; Li, Huilin ; Loo, Joseph A. ; Marty, Michael T. ; Paša-Tolić, Ljiljana ; Sandoval, Wendy ; Lermyte, Frederik
• Description:
  The combination of native electrospray ionization with top-down fragmentation in mass spectrometry (MS) allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and cofactors. Although this approach is powerful, both native MS and top-down MS are not yet well standardized, and only a limited number of laboratories regularly carry out this type of research. To address this challenge, the Consortium for Top-Down Proteomics initiated a study to develop and test protocols for native MS combined with top-down fragmentation of proteins and protein complexes across 11 instruments in nine laboratories. Here we report the summary of the outcomes to provide robust benchmarks and a valuable entry point for the scientific community.
• Subjects:
  Humans Mass Spectrometry Proteins Proteomics Spectrometry, Mass, Electrospray Ionization
• Source:
  Nat Methods. 21(12):2388-2396
• Pubmed ID:
  38744918
• Pubmed Central ID:
  PMC11561160
• Document Type:
  Journal Article
• Funding:
  S10 RR028893/RR/NCRR NIH HHSUnited States/ ; R01GM103479/U.S. Department of Health & Human Services ; National Institutes of Health (NIH)/ ; LOEWE - TRABITA/Hessisches Ministerium für Wissenschaft und Kunst (Hessen State Ministry of Higher Education, Research and the Arts)/ ; S10 OD018475/CD/ODCDC CDC HHSUnited States/ ; R01 GM125085/GM/NIGMS NIH HHSUnited States/ ; DEFC02-02ER63421/U.S. Department of Energy (DOE)/ ; R35 GM145286/GM/NIGMS NIH HHSUnited States/ ; S10 OD018475/OD/NIH HHSUnited States/ ; DE-AC05-76RL01830/DOE ; SC ; Biological and Environmental Research (BER)/ ; R35 GM128624/GM/NIGMS NIH HHSUnited States/ ; P41 GM108569/GM/NIGMS NIH HHSUnited States/ ; R01 GM103479/GM/NIGMS NIH HHSUnited States/ ; GM007185/U.S. Department of Health & Human Services ; National Institutes of Health (NIH)/ ; T32 GM007185/GM/NIGMS NIH HHSUnited States/ ; S10RR028893/U.S. Department of Health & Human Services ; National Institutes of Health (NIH)/ ; 461372424/Deutsche Forschungsgemeinschaft (German Research Foundation)/ ; R35GM145286/U.S. Department of Health & Human Services ; National Institutes of Health (NIH)/ ; 524226614/Deutsche Forschungsgemeinschaft (German Research Foundation)/
• Volume:
  21
• Issue:
  12
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:2ae3b7b3613d32e99d965612adbea03b1b9a5cdfdac2d89378c28d8acb2c87f512ac83cd469748905f27bc9103bbd90c991184a422423a1588b76c55e0caaef8
• Download URL:
  https://stacks.cdc.gov/view/cdc/169992/cdc_169992_DS1.pdf
• File Type:
  [PDF - 1.13 MB ]