Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Delhaes, Laurence ; Harun, Azian ; Chen, Sharon C.A. ; Nguyen, Quoc ; Slavin, Monica ; ... More +
Delhaes, Laurence ; Harun, Azian ; Chen, Sharon C.A. ; Nguyen, Quoc ; Slavin, Monica ; Heath, Christopher H. ; Maszewska, Krystyna ; Halliday, Catriona ; Robert, Vincent ; Sorrell, Tania C. ; Meyer, Wieland ; Less -
Corporate Authors:
Australian Scedosporium (AUSCEDO) Study Group ;
Description:
One hundred clinical isolates from a prospective nationwide study of scedosporiosis in Australia (2003-2005) and 46 additional isolates were genotyped by internal transcribed spacer-restriction fragment length polymorphism (ITS-RFLP) analysis, ITS sequencing, and M13 PCR fingerprinting. ITS-RFLP and PCR fingerprinting identified 3 distinct genetic groups. The first group corresponded to Scedosporium prolificans (n = 83), and the other 2 comprised isolates previously identified as S. apiospermum: one of these corresponded to S. apiospermum (n = 33) and the other to the newly described species S. aurantiacum (n = 30). Intraspecies variation was highest for S. apiospermum (58%), followed by S. prolificans (45%) and S. aurantiacum (28%) as determined by PCR fingerprinting. ITS sequence variation of 2.2% was observed among S. apiospermum isolates. No correlation was found between genotype of strains and their geographic origin, body site from which they were cultured, or colonization versus invasive disease. Twelve S. prolificans isolates from 2 suspected case clusters were examined by amplified fragment length polymorphism analysis. No specific clusters were confirmed.
Subject:
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AFLP
Australia
DNA, Fungal
DNA, Ribosomal Spacer
DNA Fingerprinting
Female
Genetic Variation
Genotype
Humans
ITS-RFLP
ITS-sequencing
Male
Molecular Epidemiology
Mycetoma
Mycological Typing Techniques
PCR Fingerprinting
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Research
Scedosporium
Scedosporium Apiospermum
Scedosporium Aurantiacum
Scedosporium Prolificans
Molecular Epidemiology

Source:
Emerg Infect Dis. 14(2):282-290.
Document Type:
Journal Article ;
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:3562080c1bb1459497f92222f89cb079890d2bb33af7e78f54373f754501dd0e
File Type:

Supporting Files

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