Determination of Fentanyl Analogue Exposure Using Dried Blood Spots with LC-MS/MS

Details

• Alternative Title:
  J Anal Toxicol
• Personal Author:
  Seymour, Craig ; Shaner, Rebecca L. ; Feyereisen, Melanie C. ; Wharton, Rebekah E. ; Kaplan, Pearl ; Hamelin, Elizabeth I. ; Johnson, Rudolph C.
• Description:
  Fentanyl, and the numerous drugs derived from it, are contributing to the opioid overdose epidemic currently underway in the USA. To identify human exposure to these growing public health threats, an LC-MS-MS method for 5 μL dried blood spots (DBS) was developed. This method was developed to detect exposure to 3-methylfentanyl, alfentanil, α-methylfentanyl, carfentanil, fentanyl, lofentanil, sufentanil, norcarfentanil, norfentanyl, norlofentanil, norsufentanil, and using a separate LC-MS-MS injection, cyclopropylfentanyl, acrylfentanyl, 2-furanylfentanyl, isobutyrylfentanyl, ocfentanil and methoxyacetylfentanyl. Preparation of materials into groups of compounds was used to accommodate an ever increasing need to incorporate newly identified fentanyls. This protocol was validated within a linear range of 1.00-100 ng/mL, with precision ≤12% CV and accuracy ≥93%, as reported for the pooled blood QC samples, and limits of detection as low as 0.10 ng/mL. The use of DBS to assess fentanyl analog exposures can facilitate rapid sample collection, transport, and preparation for analysis that could enhance surveillance and response efforts in the ongoing opioid overdose epidemic.
• Subjects:
  Analgesics, Opioid Autopsy Chromatography, Liquid Data Accuracy Dried Blood Spot Testing Drug Overdose Fentanyl Furans Hematocrit Humans Humidity Illicit Drugs Substance Abuse Detection Tandem Mass Spectrometry United States

  More +
• Source:
  J Anal Toxicol. 43(4):266-276
• Pubmed ID:
  30462229
• Pubmed Central ID:
  PMC11304352
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  43
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:253547275d49cf2770bbfef45f564759e9bb9066c763e43e932f1568a1207bf05324146ff3802330c7a046dca1c4bce8b8150cdfd5e6c548a714bd023c643835
• Download URL:
  https://stacks.cdc.gov/view/cdc/159741/cdc_159741_DS1.pdf
• File Type:
  [PDF - 949.59 KB ]