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Isolation and Identification of Salmonella Species in Public Health Laboratories
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May 2024
[PDF-876.82 KB]
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Alternative Title:APHL [Isolation and Identification of Salmonella in PHLs]
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Description:The following is a procedure for the culture and isolation of Salmonella spp. from human feces. Salmonella is one of the most common etiologic agents of bacterial diarrhea. Sensitive and specific laboratory methods for the isolation,
identification, serotyping and subtyping of Salmonella are key to monitoring and control programs. Culture-independent diagnostic tests (CIDTs) have impacted the ability of public health laboratories (PHLs) to obtain bacterial isolates to perform additional characterization to identify and prevent foodborne illness outbreaks. In recent years, PHLs have received an increasing volume of specimens that tested positive for foodborne pathogens using CIDTs at the clinical laboratory. In this instance, the PHL needs to perform culture to isolate the pathogen so the isolated bacteria can be characterized by whole genome sequencing (WGS) or other methods. The goal of this document is to provide PHLs with methods to isolate and identify Salmonella spp. from stool specimens as efficiently as possible.
Running title: APHL [Isolation and Identification of Salmonella in PHLs]
This project was 100% funded with federal funds from a federal program of $1,272,683. This publication was supported by Cooperative Agreement #NU60OE000104 from the US Centers for Disease Control and Prevention (CDC). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of CDC.
Isolation-Identification-Salmonella-PHLs.pdf
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Pages in Document:8 numbered pages
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