Novel Flavivirus or New Lineage of West Nile Virus, Central Europe

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Bakonyi, Tamás ; Hubálek, Zdenek ; Rudolf, Ivo ; Nowotny, Norbert
• Description:
  A flavivirus (strain 97-103) was isolated from Culex pipens mosquitoes in 1997 following floods in South Moravia, Czech Republic. The strain exhibited close antigenic relationship to West Nile virus (WNV) prototype strain Eg-101 in a cross-neutralization test. In this study, mouse pathogenicity characteristics and the complete nucleotide and putative amino acid sequences of isolate 97-103, named Rabensburg virus (RabV) after a nearby Austrian city, were determined. RabV shares only 75%-77% nucleotide identity and 89%-90% amino acid identity with representative strains of WNV lineages 1 and 2. Another RabV strain (99-222) was isolated in the same location 2 years later; it showed >99% nucleotide identity to strain 97-103. Phylogenetic analyses of RabV, WNV strains, and other members of the Japanese encephalitis virus (JEV) complex clearly demonstrated that RabV is either a new (third) lineage of WNV or a novel flavivirus of the JEV group.
• Subjects:
  Amino Acid Sequence Animals Animals, Suckling Base Sequence Complete Genome Analysis Czech Republic Female Flaviviridae Flavivirus Flavivirus Infections Mice Mice, Inbred ICR Molecular Sequence Data Phylogenetic Analysis Phylogeny Rabensburg Virus Research Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sequence Alignment Specific Pathogen-Free Organisms Virulence West Nile Virus

  More +
• Source:
  Emerg Infect Dis. 11(2):225-231.
• Document Type:
  Journal Article
• Volume:
  11
• Issue:
  2
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:02c578bb85f10f34b9ab6e2914d5e1952e72f1031ed1d6230325ba26e2642c14
• Download URL:
  https://stacks.cdc.gov/view/cdc/15737/cdc_15737_DS1.pdf
• File Type:
  [PDF - 292.43 KB ]