Lung-delivered IL-10 Therapy Elicits Beneficial Effects Via Immune Modulation in Organic Dust Exposure-induced Lung Inflammation

Schwab, Aaron D.; Wyatt, Todd A.; Nelson, Amy J.; Gleason, Angela; Gaurav, Rohit; Romberger, Debra J.; Poole, Jill A.

doi:10.1080/1547691X.2024.2332172

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Lung-delivered IL-10 Therapy Elicits Beneficial Effects Via Immune Modulation in Organic Dust Exposure-induced Lung Inflammation

12 2024
By Schwab, Aaron D. ; Wyatt, Todd A. ; Nelson, Amy J. ; ...
Source: J Immunotoxicol. 21(1):2332172

Public Access Version Available on: December 01, 2025, 12:00 AM

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English

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Details:

Alternative Title:

J Immunotoxicol
Personal Author:

Schwab, Aaron D. ; Wyatt, Todd A. ; Nelson, Amy J. ; Gleason, Angela ; Gaurav, Rohit ; ... More +

Schwab, Aaron D. ; Wyatt, Todd A. ; Nelson, Amy J. ; Gleason, Angela ; Gaurav, Rohit ; Romberger, Debra J. ; Poole, Jill A. Less -
Description:

Efficacious therapeutic options capable of resolving inflammatory lung disease associated with environmental and occupational exposures are lacking. This study sought to determine the preclinical therapeutic potential of lung-delivered recombinant interleukin (IL)-10 therapy following acute organic dust exposure in mice. Here, C57BL/6J mice were intratracheally instilled with swine confinement organic dust extract (ODE) (12.5%, 25%, 50% concentrations) with IL-10 (1 μg) treatment or vehicle control intratracheally-administered three times: 5 hr post-exposure and then daily for 2 days. The results showed that IL-10 treatment reduced ODE (25%)-induced weight loss by 66% and 46% at Day 1 and Day 2 post-exposure, respectively. IL-10 treatment reduced ODE (25%, 50%)-induced lung levels of TNFα (-76%, -83% [reduction], respectively), neutrophil chemoattractant CXCL1 (-51%, -60%), and lavage fluid IL-6 (-84%, -89%). IL-10 treatment reduced ODE (25%, 50%)-induced lung neutrophils (-49%, -70%) and recruited CD11c|CD11b| monocyte-macrophages (-49%, -70%). IL-10 therapy reduced ODE-associated expression of antigen presentation (MHC Class II, CD80, CD86) and inflammatory (Ly6C) markers and increased anti-inflammatory CD206 expression on CD11c|CD11b| cells. ODE (12.5%, 25%)-induced lung pathology was also reduced with IL-10 therapy. In conclusion, the studies here showed that short-term, lung-delivered IL-10 treatment induced a beneficial response in reducing inflammatory consequences (that were also associated with striking reduction in recruited monocyte-macrophages) following acute complex organic dust exposure.
Subjects:

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Animals Dust Interleukin-10 Lung Lung Diseases Mice Mice, Inbred C57BL Pneumonia Swine
Keywords:

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Immunology Inflammation Lung Disease Organic Dust Therapy
Source:

J Immunotoxicol. 21(1):2332172
Pubmed ID:

38563602
Pubmed Central ID:

PMC11137733
Document Type:

Journal Article
Funding:

IK6 BX005962/BX/BLRD VAUnited States/ ; F30 ES036063/ES/NIEHS NIH HHSUnited States/ ; P50 AA030407/AA/NIAAA NIH HHSUnited States/ ; U54 OH010162/OH/NIOSH CDC HHSUnited States/ ; R01 OH012045/OH/NIOSH CDC HHSUnited States/ ; ... More +

IK6 BX005962/BX/BLRD VAUnited States/ ; F30 ES036063/ES/NIEHS NIH HHSUnited States/ ; P50 AA030407/AA/NIAAA NIH HHSUnited States/ ; U54 OH010162/OH/NIOSH CDC HHSUnited States/ ; R01 OH012045/OH/NIOSH CDC HHSUnited States/ ; I01 BX005886/BX/BLRD VAUnited States/ Less -
Volume:

21
Issue:

1
Collection(s):

CDC Public Access
Main Document Checksum:

[+]

urn:sha-512:06eb54a5f4fc6cfdbad5bcf9645d21bca818f9ba5fb8b734afbe5d553eae1ac7221ccad8f9715b305fc0e42aaa24bd4250e27968580af22061f20425919b292b
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