Follow-up of humoral immune response after HPV vaccination using first-void urine: a longitudinal cohort study

Details

• Alternative Title:
  J Med Virol
• Personal Author:
  Téblick, Laura ; Pattyn, Jade ; Van Keer, Severien ; De Smet, Annemie ; De Coster, Ilse ; Tjalma, Wiebren A. A. ; Rajbhandari, Ira ; Panicker, Gitika ; Unger, Elizabeth R. ; Vorsters, Alex
• Description:
  Assessment of humoral immune responses following human papillomavirus (HPV) vaccination currently relies on invasive blood sampling. This longitudinal cohort study explores the usability of first-void urine as a noninvasive alternative sample for antibody detection. In this study, 58 women receiving three doses of the 9vHPV vaccine within a Gardasil9 (9vHPV) Phase III randomized controlled trial were included. Participants provided paired first-void urine and blood samples before vaccination (M0), 1 month after the third dose (M7), and ~3 years after the third dose (M43). Type-specific antibody responses to the 9vHPV types were analyzed in 174 first-void urine and 172 serum samples using a virus-like particle-based IgG multiplex enzyme-linked immunosorbent assay. Additionally, total human IgG concentrations were determined using the BioPlex assay. At M7, 1 month after complete 9vHPV vaccination, 95%-100% of first-void urine and 100% of serum samples had detectable concentrations, varying by HPV type. At M43, 84%-100% of first-void urine and 98%-100% of serum samples had HPV-specific antibody concentrations. Results show significant Spearman rank correlations between type-specific HPV-antibody concentrations for paired first-void urine and serum at all time points. This study confirms the potential feasibility of utilizing first-void urine as a noninvasive immunological sample within HPV vaccine trials.
• Subjects:
  Antibodies, Viral Female Follow-Up Studies Humans Immunity, Humoral Immunoglobulin G Longitudinal Studies Papillomavirus Infections Papillomavirus Vaccines Vaccination
• Keywords:
  Antibody First-void Urine HPV Human Papillomavirus Humoral Immunity
• Source:
  J Med Virol. 95(10):e29133
• Pubmed ID:
  37812015
• Pubmed Central ID:
  PMC11057001
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  95
• Issue:
  10
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:5bf70a5a002a75dfc7f2508b15b91bf624b9037cbb7492df0abace0c834f00e9ddd8cfe7476630b453892b3702a568e1681d48a384b1306907d5b2be24e33aa2
• Download URL:
  https://stacks.cdc.gov/view/cdc/155091/cdc_155091_DS1.pdf
• File Type:
  [PDF - 1.14 MB ]