Glowstick-inspired smartphone-readable reporters for sensitive, multiplexed lateral flow immunoassays

Details

• Alternative Title:
  Commun Eng
• Personal Author:
  Brosamer, Kristen ; Kourentzi, Katerina ; Willson, Richard C. ; Vu, Binh V.
• Description:
  The COVID-19 pandemic has increased demand for point-of-care (POC) screening tests such as lateral flow assays (LFAs) and highlighted the need for sensitive and cost-effective POC diagnostic platforms. Here, we demonstrate an LFA platform using standard fluorescent nanoparticle reporters in which optical excitation is replaced by chemical excitation using the peroxyoxalate-based chemistry of inexpensive, shelf-stable glowsticks. The one-step chemi-excitation of fluorescent particles produces visible light readable by an unmodified smartphone, enhancing sensitivity while preserving simplicity and cost-effectiveness. Our Glow LFA detected the common model analyte human chorionic gonadotropin with a limit of detection (LoD) of 39 pg/mL-over ten times more sensitive than standard gold nanoparticles using the same antibodies. We also demonstrate its application to the detection of SARS-CoV-2 nucleoprotein at 100 pg/mL in nasal swab extract. Multiple fluorescent dyes can be chemi-excited by a single reagent, allowing for color multiplexing on a single LFA strip with a smartphone camera. The detection of three analytes on a single LFA test line was demonstrated using red, green, and blue fluorescent reporter particles, making glow LFA a promising platform for multiplexed detection.
• Source:
  Commun Eng. 2
• Pubmed ID:
  38586601
• Pubmed Central ID:
  PMC10955955
• Document Type:
  Journal Article
• Funding:
  R01 AR072742/AR/NIAMS NIH HHSUnited States/ ; U01CK000512/ACL/ACL HHSUnited States/ ; U01 CK000512/CK/NCEZID CDC HHSUnited States/ ; U54 EB015408/EB/NIBIB NIH HHSUnited States/ ; R61 AI174294/AI/NIAID NIH HHSUnited States/
• Volume:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:da5d508418f2b144eb4d9544ab2d7e6da60a4b6e46d34e141a8f80f0d6d6cea9
• Download URL:
  https://stacks.cdc.gov/view/cdc/154067/cdc_154067_DS1.pdf
• File Type:
  [PDF - 1.41 MB ]