Comparison of Zika virus inactivation methods for reagent production and disinfection methods

Details

• Alternative Title:
  J Virol Methods
• Personal Author:
  Chida, Asiya S. ; Goldstein, Jason M. ; Lee, Joo ; Tang, Xiaoling ; Bedi, Kanwar ; Herzegh, Owen ; Moon, Jonathan L. ; Petway, David ; Bagarozzi, Dennis A. ; Hughes, Laura J.
• Description:
  Zika virus (ZIKV) infection remains a public health concern necessitating demand for long-term virus production for diagnostic assays and R&D activities. Inactivated virus constitutes an important component of the Trioplex rRT-PCR assay and serological IgM assay (MAC-ELISA). The aim of our study is to establish standard methods of ZIKV inactivation while maintaining antigenicity and RNA integrity. We tested viral supernatants by four different inactivation methods: 1. Heat inactivation at 56 °C and 60 °C; 2. Gamma-Irradiation; 3. Chemical inactivation by Beta-propiolactone (BPL) and 4. Fast-acting commercial disinfecting agents. Effectivity was measured by cytopathic effect (CPE) and plaque assay. RNA stability and antigenicity were measured by RT-PCR and MAC-ELISA, respectively. Results: Heat inactivation: Low titer samples, incubated at 56 °C for 2 h, showed neither CPE or plaques compared to high titer supernatants that required 2.5 h. Inactivation occurred at 60 °C for 60 min with all virus titers. Gamma irradiation: Samples irradiated at ≥3 Mrad for low virus concentrations and ≥5Mrad for high virus titer completely inactivated virus. Chemical Inactivation: Neither CPE nor plaques were observed with ≥0.045 % BPL inactivation of ZIKV. Disinfectant: Treatment of viral supernatants with Micro-Chem Plus™, inactivated virus in 2 min, whereas, Ethanol (70 %) and STERIS Coverage® Spray TB inactivated the virus in 5 min.
• Source:
  J Virol Methods. 287:114004
• Document Type:
  Journal Article
• Volume:
  287
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:296ff57eba185be7ade89e300ad7d93cb8264397b7c417005a9a82ede63c6e45cdda410d3b1d471df34371990bf607f9a67f6a8a237478071a064fdde419a933
• Download URL:
  https://stacks.cdc.gov/view/cdc/150374/cdc_150374_DS1.pdf
• File Type:
  [PDF - 597.08 KB ]