Human Metapneumovirus Detection in Patients with Severe Acute Respiratory Syndrome
Published Date:Sep 2003
Source:Emerg Infect Dis. 9(9):1058-1063.
Communicable Diseases, Emerging
Reverse Transcriptase Polymerase Chain Reaction
Severe Acute Respiratory Syndrome
Description:We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected with HMPV; 6 of these 25 patients were also infected with coronavirus, and another 5 patients (10.4%) were infected with coronavirus alone. Using this combination approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidney (LLC-MK2) cells commonly used in previous studies for isolation of HMPV. These widely available HEp-2 cells should be included in conjunction with a molecular method for cell culture followup to detect HMPV, particularly in patients with SARS.
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