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Evaluation of library preparation methods for Illumina next generation sequencing of small amounts of DNA from foodborne parasites
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11 2016
Source: J Microbiol Methods. 130:23-26 -
Alternative Title:J Microbiol Methods
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Description:Illumina library preparation methods for ultra-low input amounts were compared using genomic DNA from two foodborne parasites (Angiostrongylus cantonensis and Cyclospora cayetanensis) as examples. The Ovation Ultralow method resulted in libraries with the highest concentration and produced quality sequencing data, even when the input DNA was in the picogram range.
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Pubmed ID:27553132
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Pubmed Central ID:PMC10750217
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