Evaluation of library preparation methods for Illumina next generation sequencing of small amounts of DNA from foodborne parasites

Details

• Alternative Title:
  J Microbiol Methods
• Personal Author:
  Nascimento, Fernanda S. ; Wei-Pridgeon, Yuping ; Arrowood, Michael J. ; Moss, Delynn ; da Silva, Alexandre J. ; Talundzic, Eldin ; Qvarnstrom, Yvonne
• Description:
  Illumina library preparation methods for ultra-low input amounts were compared using genomic DNA from two foodborne parasites (Angiostrongylus cantonensis and Cyclospora cayetanensis) as examples. The Ovation Ultralow method resulted in libraries with the highest concentration and produced quality sequencing data, even when the input DNA was in the picogram range.
• Keywords:
  • Next generation sequencing
  • Illumina
  • MiSeq
  • Library preparation
  • Angiostrongylus cantonensis
  • Cyclospora cayetanensis
  • Angiostrongylus cantonensis
  • Animals
  • Base Sequence
  • Cyclospora
  More +
• Source:
  J Microbiol Methods. 130:23-26
• Pubmed ID:
  27553132
• Pubmed Central ID:
  PMC10750217
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  130
• Download URL:
  https://stacks.cdc.gov/view/cdc/138377/cdc_138377_DS1.pdf
• File Type:
  [PDF - 467.41 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:59cb91be4177d70d2bf0f3f92cc8ff9f3c5d737b524439f5358cca6b48b2f243