Evaluation of library preparation methods for Illumina next generation sequencing of small amounts of DNA from foodborne parasites

Nascimento, Fernanda S.; Wei-Pridgeon, Yuping; Arrowood, Michael J.; Moss, Delynn; da Silva, Alexandre J.; Talundzic, Eldin; Qvarnstrom, Yvonne

doi:10.1016/j.mimet.2016.08.020

i

Evaluation of library preparation methods for Illumina next generation sequencing of small amounts of DNA from foodborne parasites

Supporting Files

11 2016
By Nascimento, Fernanda S. ; Wei-Pridgeon, Yuping ; Arrowood, Michael J. ; ...

File Language:

English

Details

Alternative Title:

J Microbiol Methods
Personal Author:

Nascimento, Fernanda S. ; Wei-Pridgeon, Yuping ; Arrowood, Michael J. ; Moss, Delynn ; da Silva, Alexandre J. ; Talundzic, Eldin ; Qvarnstrom, Yvonne
Description:

Illumina library preparation methods for ultra-low input amounts were compared using genomic DNA from two foodborne parasites (Angiostrongylus cantonensis and Cyclospora cayetanensis) as examples. The Ovation Ultralow method resulted in libraries with the highest concentration and produced quality sequencing data, even when the input DNA was in the picogram range.
Keywords:

Angiostrongylus Cantonensis Animals Base Sequence Cyclospora Cyclospora Cayetanensis DNA, Protozoan Foodborne Diseases Food Parasitology Gene Library Genes, Protozoan Genome, Protozoan High-Throughput Nucleotide Sequencing Illumina Library Preparation MiSeq Models, Biological Next Generation Sequencing Parasites
Source:

J Microbiol Methods. 130:23-26
Pubmed ID:

27553132
Pubmed Central ID:

PMC10750217
Document Type:

Journal Article
Funding:

CC999999/ImCDC/Intramural CDC HHSUnited States/
Volume:

130
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:59cb91be4177d70d2bf0f3f92cc8ff9f3c5d737b524439f5358cca6b48b2f243
Download URL:

https://stacks.cdc.gov/view/cdc/138377/cdc_138377_DS1.pdf
File Type:

[PDF - 467.41 KB ]

File Language:

English

ON THIS PAGE

Details Supporting Files

CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.

As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.