CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.
As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.
i
CDC Mumps rRT-PCR Assay vs 2 for the Detection of Mumps Virus RNA in Clinical Samples
-
08/20/2021
[PDF-156.04 KB]
Details:
-
Corporate Authors:
-
Description:Version 3.0 08/20/21
Changes compared to version 2: Version 2 listed incorrect cycling conditions. Version 3 lists the correct condition. The change is highlighted in green.
This rRT-PCR assay is designed to detect both mumps vaccine and wild type strains. This assay targets the mumps N- gene. Version 1 of the assay (Rota et al., Clin. Vaccine Immunol. 20(3) 2013 p391) occasionally produced false-negative results with specimens in which the mumps forward primer and probe binding sites had nucleotide substitutions. The new version of the assay improves detection in these specimens through introduction of degenerate positions in the forward primer and probe. In addition, the enzyme mix was changed to the QuantiTect kit to allow use of the same enzyme that is used for the CDC measles and MeVA rRT-PCR assays. The reagents, instruments, and other information listed below have been validated by CDC for use in their CLIA-approved assays, but each laboratory should conduct their own validation and establish their own SOPs.
-
Subjects:
-
Document Type:
-
Genre:
-
CIO:
-
Division:
-
Pages in Document:3 unnumbered pages
-
Collection(s):
-
Main Document Checksum:
-
Download URL:
-
File Type:
