A highly expressed odorant receptor from the yellow fever mosquito, AaegOR11, responds to (+)- and (−)-fenchone and a phenolic repellent

Details

• Alternative Title:
  Insect Biochem Mol Biol
• Personal Author:
  Lu, WeiYu ; Leal, Walter S. ; Brisco, Katherine K. ; An, Sunny ; Cornel, Anthony J.
• Description:
  The cornerstone of the reverse chemical ecology approach is the identification of odorant receptors (OR) sensitive to compounds in a large panel of odorants. In this approach, we de-orphanize ORs and, subsequently, measure behaviors elicited by these semiochemicals. After that, we evaluate behaviorally active compounds for applications in insect vector management. Intriguingly, multiple ORs encoded by genes highly expressed in mosquito antennae do not respond to any test odorant. One such case is CquiOR125 from the southern house mosquito, Culex quinquefasciatus Say. To better understand CquiOR125's role in Culex mosquito olfaction, we have cloned a CquiOR125 orthologue in the genome of the yellow fever mosquito, Aedes aegypti (L.), AaegOR11. Unlike the unresponsive nature of the orthologue in Cx. quinquefasciatus, oocytes co-expressing AaegOR11 and AaegOrco elicited robust responses when challenged with fenchone, 2,3-dimethylphenol, 3,4-dimethylphenol, 4-methycyclohexanol, and acetophenone. Interestingly, AaegOR11 responded strongly and equally to (+)- and (-)-fenchone, with no chiral discrimination. Contrary to reports in the literature, fenchone did not show any repellency activity against Ae. aegypti or Cx. quinquefasciatus. Laboratory and field tests did not show significant increases in egg captures in cups filled with fenchone solutions compared to control cups. The second most potent ligand, 2,3-dimethylphenol, showed repellency activity stronger than that elicited by DEET at the same dose. We, therefore, concluded that AaegOR11 is a mosquito repellent sensor. It is feasible that CquiOR125 responds to repellents that remain elusive.
• Keywords:
  2,3-dimethylphenol AaegOR84 AaegOR113 Aedes Aedes Aegypti Animals CquiOR125 Culex Culex Quinquefasciatus Insect Proteins Insect Repellents Mosquito Vectors Receptors, Odorant Yellow Fever

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• Source:
  Insect Biochem Mol Biol. 151:103866
• Pubmed ID:
  36347453
• Pubmed Central ID:
  PMC10540613
• Document Type:
  Journal Article
• Funding:
  R01 AI095514/AI/NIAID NIH HHSUnited States/ ; U01CK000516/ACL/ACL HHSUnited States/ ; U01 CK000516/CK/NCEZID CDC HHSUnited States/
• Volume:
  151
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:5667adfd859dbf15901d0714314595f1b9f7c710968c384b1dae58c9ba1303e2
• Download URL:
  https://stacks.cdc.gov/view/cdc/133534/cdc_133534_DS1.pdf
• File Type:
  [PDF - 1.86 MB ]