Validation of a diphtheria toxoid multiplex bead assay for serosurveys

Details

• Alternative Title:
  Diagn Microbiol Infect Dis
• Personal Author:
  Scobie, Heather M. ; Khetsuriani, Nino ; Efstratiou, Androulla ; Priest, Jeffrey W.
• Description:
  We validated a multiplex bead assay for diphtheria toxoid IgG antibodies against the Vero cell toxin neutralization test using 1300 specimens (correlation = 0.88). At the ≥0.01 IU/mL cutoff for minimal seroprotection, sensitivity was 95% and specificity was 83%. Agreement for three categories (<0.01, 0.01-<0.1, ≥0.1 IU/mL) was 81% (kappa = 0.71).
• Subjects:
  Animals Antibodies, Bacterial Chlorocebus Aethiops Diphtheria Toxoid Immunoglobulin G Neutralization Tests Reproducibility Of Results Sensitivity And Specificity Serologic Tests Vero Cells
• Source:
  Diagn Microbiol Infect Dis. 100(3):115371
• Pubmed ID:
  33838594
• Pubmed Central ID:
  PMC10370405
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  100
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:91c01c69b67b0ef9478dc1296c5c97e1c33101adf32f430ef3c438b10961d77e
• Download URL:
  https://stacks.cdc.gov/view/cdc/131472/cdc_131472_DS1.pdf
• File Type:
  [PDF - 271.74 KB ]