i
Aging influence on pulmonary and systemic inflammation and neural metabolomics arising from pulmonary multi-walled carbon nanotube exposure in apolipoprotein E-deficient and C57BL/6 female mice
-
2023
-
Details:
-
Alternative Title:figshare Academic Research System
-
Journal Article:Inhalation Toxicology
-
Personal Author:
-
Corporate Authors:Center for Inhalation Toxicology, West Virginia University ; Center for Translational Science, Florida International University ; College of Health Solutions, Arizona State University ; College of Nursing, University of New Mexico ; Department of Anatomy and Neurobiology, Virginia Commonwealth University ; ... More +
-
Description:Environmental exposures exacerbate age-related pathologies, such as cardiovascular and neurodegenerative diseases. Nanoparticulates, and specifically carbon nanomaterials, are a fast-growing contributor to the category of inhalable pollutants, whose risks to health are only now being unraveled. The current study assessed the exacerbating effect of age on multiwalled-carbon nanotube (MWCNT) exposure in young and old C57BL/6 and ApoE−/− mice. Female C57BL/6 and apolipoprotein E-deficient (ApoE−/−) mice, aged 8 weeks and 15 months, were exposed to 0 or 40 µg MWCNT via oropharyngeal aspiration. Pulmonary inflammation, inflammatory bioactivity of serum, and neurometabolic changes were assessed at 24 h post-exposure. Pulmonary neutrophil infiltration was induced by MWCNT in bronchoalveolar lavage fluid in both C57BL/6 and ApoE−/−. Macrophage counts decreased with MWCNT exposure in ApoE−/− mice but were unaffected by exposure in C57BL/6 mice. Older mice appeared to have greater MWCNT-induced total protein in lavage fluid. BALF cytokines and chemokines were elevated with MWCNT exposure, but CCL2, CXCL1, and CXCL10 showed reduced responses to MWCNT in older mice. However, no significant serum inflammatory bioactivity was detected. Cerebellar metabolic changes in response to MWCNT were modest, but age and strain significantly influenced metabolite profiles assessed. ApoE−/− mice and older mice exhibited less robust metabolite changes in response to exposure, suggesting a reduced health reserve. Age influences the pulmonary and neurological responses to short-term MWCNT exposure. However, with only the model of moderate aging (15 months) in this study, the responses appeared modest compared to inhaled toxicant impacts in more advanced aging models.
-
Content Notes:Stacks:126192
-
Keywords:
-
DOI:
-
Landing Page:
-
Modified:01/19/2023
-
Date Issued:01/01/2023
-
Document Type:
-
Collection(s):
-
Main Document Checksum: