Circulating miRs-183-5p, -206-3p and -381-3p may serve as novel biomarkers for 4,4’-methylene diphenyl diisocyanate exposure

Hettick, Justin M.; Law, Brandon F.; Lin, Chen-Chung; Siegel, Paul D.

doi:10.6084/m9.figshare.7477601

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Circulating miRs-183-5p, -206-3p and -381-3p may serve as novel biomarkers for 4,4’-methylene diphenyl diisocyanate exposure

2019
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By Hettick, Justin M. ; Law, Brandon F. ; Lin, Chen-Chung ; ...

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figshare Academic Research System
Personal Author:

Hettick, Justin M. ; Law, Brandon F. ; Lin, Chen-Chung ; Siegel, Paul D.

Hettick, Justin M. ; Law, Brandon F. ; Lin, Chen-Chung ; Siegel, Paul D. Less -
Corporate Authors:

Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health ; Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention ; Health Effects Laboratory Division, National Institute for Occupational Safety and Health

Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health ; Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention ; Health Effects Laboratory Division, National Institute for Occupational Safety and Health Less -
Description:

Background: Occupational exposure to the most widely used diisocyanate, 4,4’-methylene diphenyl diisocyanate (MDI), is a cause of occupational asthma (OA). Early recognition of MDI exposure and sensitization is essential for the prevention of MDI-OA. Objective: Identify circulating microRNAs (miRs) as novel biomarkers for early detection of MDI exposure and prevention of MDI-OA. Materials and methods: Female BALB/c mice were exposed to one of three exposure regimens: dermal exposure to 1% MDI in acetone; nose-only exposure to 4580 ± 1497 μg/m3 MDI-aerosol for 60 minutes; or MDI dermal exposure/sensitization followed by MDI-aerosol inhalation challenge. Blood was collected and miRCURY™ miRs qPCR Profiling Service was used to profile circulate miRs from dermally exposed mice. Candidate miRs were identified and verified from mice exposed to three MDI-exposure regimens by TaqMan® miR assays. Results: Up/down-regulation patterns of circulating mmu-miRs-183-5p, -206-3p and -381-3p were identified and verified. Circulating mmu-miR-183-5p was upregulated whereas mmu-miRs-206-3p and -381-3p were downregulated in mice exposed via all three MDI exposure regimens. Discussion and conclusion: Upregulation of circulating miR-183-5p along with downregulation of circulating miRs-206-3p and -381-3p may serve as putative biomarkers of MDI exposure and may be considered as potential candidates for validation in exposed human worker populations.
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29999 Physical Sciences Not Elsewhere Classified 39999 Chemical Sciences Not Elsewhere Classified 59999 Environmental Sciences Not Elsewhere Classified 69999 Biological Sciences Not Elsewhere Classified Biochemistry Biophysics Cell Biology FOS: Biological Sciences FOS: Chemical Sciences FOS: Clinical Medicine FOS: Earth And Related Environmental Sciences FOS: Physical Sciences Genetics Immunology Molecular Biology Pharmacology Science Policy
DOI:

https://dx.doi.org/10.6084/m9.figshare.7477601
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https://tandf.figshare.com/articles/Circulating_miRs-183-5p_-206-3p_and_-381-3p_may_serve_as_novel_biomarkers_for_4_4_-methylene_diphenyl_diisocyanate_exposure/7477601
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11/26/2019
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National Institute for Occupational Safety and Health (NIOSH)
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National Institute for Occupational Safety and Health
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Circulating miRs-183-5p, -206-3p and -381-3p may serve as novel biomarkers for 4,4’-methylene diphenyl diisocyanate exposure

[PDF - 1.87 MB]

Personal Author:

Lin, Chen-Chung ;

Law, Brandon F.
...

December 12 2018 | Biomarkers. 24(1):76-90

Description:

Background:Occupational exposure to the most widely used diisocyanate, 4,4’-methylene diphenyl diisocyanate (MDI), is a cause of occupational asthma...

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[PDF - 1.87 MB]

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[PDF - 1.87 MB]

Circulating miRs-183-5p, -206-3p and -381-3p may serve as novel biomarkers for 4,4’-methylene diphenyl diisocyanate exposure

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