Evaluation of a Rapid Detection Influenza Virus A Antigens Kit Using Paired Serum Antibody Test

Details

• Alternative Title:
  Yonsei Medical Journal
• Personal Author:
  Chen, Hangwei ; Wang, Huimin ; Li, Xuehui ; You, Lanhua ; Wei, Juan ; Shi, Weixian
• Description:
  Purpose
  
  To evaluate the feasibility for gold immunochromatographic assay (GICA) in rapid detection of influenza virus A infection.
  
  Materials and Methods
  
  Seventy-three patients were enrolled. All patients contributed nasopharyngeal secretions and paired serum samples. Nasopharyngeal secretions was used for colloidal gold immunochromatographic rapid assay for influenza A virus immediately after the collection of specimen. Paired serum samples were used for the hemagglutination inhibition assay at the Centers for Disease Control and Prevention influenza network laboratory in Beijing.
  
  Results
  
  Compare GICA test to hemagglutination inhibition (HI) assay, the Kappa value was 0.402 and the p value in the paired χ2 test was higher than 0.05. Therefore, the difference was not statistically significant. The sensitivity of GICA was 50.0% and the specificity was 90.2%, and the negative predictive value was 90.2%.
  
  Conclusion
  
  The sensitivity for Influenza A antigen detection by using GICA is relatively low, the specificity is relatively satisfactory.
  
  
• Subjects:
  Antibody Colloidal Gold Immunochromatographic Assay Influenza A Virus Original Article Rapid Test
• Source:
  Yonsei Medical Journal. 2013; 54(2):476-479.
• DOI:
  http://dx.crossref.org/10.3349/ymj.2013.54.2.476
• Document Type:
  Journal Article
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:213ee850ac3a7fe1b5d0da3faca9ff6411328a861bfce50068fd41b57cd9a84f4f1f4e71f40c6825d4a553ae745fcb32ecf2ecc6901e210cf8a771aec7314164
• Download URL:
  https://stacks.cdc.gov/view/cdc/12454/cdc_12454_DS1.pdf
• File Type:
  [PDF - 562.35 KB ]