Metabolomic Profiling of Fatty Acid and Amino Acid Metabolism in Youth With Obesity and Type 2 Diabetes

Details

• Alternative Title:
  Diabetes Care
• Personal Author:
  Mihalik, Stephanie J. ; Michaliszyn, Sara F. ; de las Heras, Javier ; Bacha, Fida ; Lee, SoJung ; Chace, Donald H. ; DeJesus, Victor R. ; Vockley, Jerry ; Arslanian, Silva A.
• Description:
  OBJECTIVE ; We compared acylcarnitine (AcylCN) species, common amino acid and fat oxidation (FOX) byproducts, and plasma amino acids in normal weight (NW; n = 39), obese (OB; n = 64), and type 2 diabetic (n = 17) adolescents. ; RESEARCH DESIGN AND METHODS ; Fasting plasma was analyzed by tandem mass spectrometry, body composition by dual energy X-ray absorptiometry and computed tomography, and total-body lipolysis and substrate oxidation by 2H5glycerol and indirect calorimetry, respectively. In vivo insulin sensitivity (IS) was assessed with a 3-h hyperinsulinemic-euglycemic clamp. ; RESULTS ; Long-chain AcylCNs (C18:2-CN to C14:0-CN) were similar among the three groups. Medium- to short-chain AcylCNs (except C8 and C10) were significantly lower in type 2 diabetes compared with NW, and when compared with OB, C2-, C6-, and C10-CN were lower. Amino acid concentrations were lower in type 2 diabetes compared with NW. Fasting lipolysis and FOX were higher in OB and type 2 diabetes compared with NW, and the negative association of FOX to C10:1 disappeared after controlling for adiposity, Tanner stage, and sex. IS was lower in OB and type 2 diabetes with positive associations between IS and arginine, histidine, and serine after adjusting for adiposity, Tanner stage, and sex. ; CONCLUSIONS ; These metabolomics results, together with the increased rates of in vivo FOX, are not supportive of defective fatty acid or amino acid metabolism in obesity and type 2 diabetes in youth. Such observations are consistent with early adaptive metabolic plasticity in youth, which over time—with continued obesity and aging—may become dysfunctional, as observed in adults.
• Subjects:
  • Original Research
  • Absorptiometry, Photon
  • Adolescent
  • Amino Acids
  • Carnitine
  • Diabetes Mellitus, Type 2
  • Fasting
  • Fatty Acids
  • Female
  • Humans
  More +
• Source:
  Diabetes Care. 2012; 35(3):605-611.
• Document Type:
  Journal Article
• Volume:
  35
• Issue:
  3
• Download URL:
  https://stacks.cdc.gov/view/cdc/12431/cdc_12431_DS1.pdf
• File Type:
  [PDF - 289.89 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:4800afd8d4f73669d82ec96557fab0cfb7ab43f7a55fd8696fd29fe46c551d30